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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas fluorescens Dihydrodipicolinate reductase (dapB) CSB-YP769541FFU
CSB-EP769541FFU
CSB-BP769541FFU
CSB-MP769541FFU
CSB-EP769541FFU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-hydroxyphenylacetate decarboxylase small subunit (hpdC) CSB-YP769542DUN
CSB-EP769542DUN
CSB-BP769542DUN
CSB-MP769542DUN
CSB-EP769542DUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus UPF0309 protein in nagA 3'region CSB-YP769543LRU
CSB-EP769543LRU
CSB-BP769543LRU
CSB-MP769543LRU
CSB-EP769543LRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA protection during starvation protein (dps) CSB-YP769544KBG
CSB-EP769544KBG
CSB-BP769544KBG
CSB-MP769544KBG
CSB-EP769544KBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alcaligenes xylosoxydans xylosoxydans Sulfoacetaldehyde acetyltransferase (xsc), partial CSB-YP769545AGR
CSB-EP769545AGR
CSB-BP769545AGR
CSB-MP769545AGR
CSB-EP769545AGR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP769546BXF
CSB-EP769546BXF
CSB-BP769546BXF
CSB-MP769546BXF
CSB-EP769546BXF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP769547CEAF
CSB-EP769547CEAF
CSB-BP769547CEAF
CSB-MP769547CEAF
CSB-EP769547CEAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica 17.7 kDa class I heat shock protein (HSP17.7) CSB-YP769548OFG
CSB-EP769548OFG
CSB-BP769548OFG
CSB-MP769548OFG
CSB-EP769548OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable protein phosphatase 2C 68 (At5g06750) CSB-YP769549DOA
CSB-EP769549DOA
CSB-BP769549DOA
CSB-MP769549DOA
CSB-EP769549DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable membrane-associated kinase regulator 6 (MAKR6) CSB-YP769550DOA
CSB-EP769550DOA
CSB-BP769550DOA
CSB-MP769550DOA
CSB-EP769550DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Nuclear transcription factor Y subunit A-7 (NFYA7) CSB-YP769551DOA
CSB-EP769551DOA
CSB-BP769551DOA
CSB-MP769551DOA
CSB-EP769551DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana NAC domain-containing protein 78 (NAC078), partial CSB-YP769552DOA
CSB-EP769552DOA
CSB-BP769552DOA
CSB-MP769552DOA
CSB-EP769552DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crocus sativus Zeaxanthin 7,8 (7',8')-cleavage dioxygenase, chromoplast (ZCD), partial CSB-YP769553CFAC
CSB-EP769553CFAC
CSB-BP769553CFAC
CSB-MP769553CFAC
CSB-EP769553CFAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycyrrhiza echinata Isoflavone 7-O-methyltransferase (D7OMT) CSB-YP769554GGW
CSB-EP769554GGW
CSB-BP769554GGW
CSB-MP769554GGW
CSB-EP769554GGW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative DNA repair protein RAD23-4 (RAD23-4) CSB-YP769555DOA
CSB-EP769555DOA
CSB-BP769555DOA
CSB-MP769555DOA
CSB-EP769555DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana attenuata 5-epi-aristolochene synthase 1 (EAS), partial CSB-YP769556NBAZ
CSB-EP769556NBAZ
CSB-BP769556NBAZ
CSB-MP769556NBAZ
CSB-EP769556NBAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica NifU-like protein 1, chloroplastic (NIFU1) CSB-YP769557OFG
CSB-EP769557OFG
CSB-BP769557OFG
CSB-MP769557OFG
CSB-EP769557OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein At1g26930 (At1g26930) CSB-YP769558DOA
CSB-EP769558DOA
CSB-BP769558DOA
CSB-MP769558DOA
CSB-EP769558DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cationic amino acid transporter 1 (CAT1), partial CSB-YP769559DOA
CSB-EP769559DOA
CSB-BP769559DOA
CSB-MP769559DOA
CSB-EP769559DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Acyl-[acyl-carrier-protein] desaturase 4, chloroplastic (Os03g0423300, LOC_Os03g30950) CSB-YP769560OFG
CSB-EP769560OFG
CSB-BP769560OFG
CSB-MP769560OFG
CSB-EP769560OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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