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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant UPF0296 protein BA_4010/GBAA_4010/BAS3723 (BA_4010, GBAA_4010, BAS3723) CSB-YP769101BQE
CSB-EP769101BQE
CSB-BP769101BQE
CSB-MP769101BQE
CSB-EP769101BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L28 (rpmB) CSB-YP769102BQE
CSB-EP769102BQE
CSB-BP769102BQE
CSB-MP769102BQE
CSB-EP769102BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L19 (rplS) CSB-YP769103BQE
CSB-EP769103BQE
CSB-BP769103BQE
CSB-MP769103BQE
CSB-EP769103BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP769104BQE
CSB-EP769104BQE
CSB-BP769104BQE
CSB-MP769104BQE
CSB-EP769104BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 1-deoxy-D-xylulose 5-phosphate reductoisomerase 2 (dxr2) CSB-YP769105BQE
CSB-EP769105BQE
CSB-BP769105BQE
CSB-MP769105BQE
CSB-EP769105BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S15 (rpsO) CSB-YP769106BQE
CSB-EP769106BQE
CSB-BP769106BQE
CSB-MP769106BQE
CSB-EP769106BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA mismatch repair protein mutL (mutL), partial CSB-YP769107BQE
CSB-EP769107BQE
CSB-BP769107BQE
CSB-MP769107BQE
CSB-EP769107BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-adenosylmethionine decarboxylase proenzyme 2 (speH2) CSB-YP769108BQE
CSB-EP769108BQE
CSB-BP769108BQE
CSB-MP769108BQE
CSB-EP769108BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triosephosphate isomerase (tpiA) CSB-YP769109BQE
CSB-EP769109BQE
CSB-BP769109BQE
CSB-MP769109BQE
CSB-EP769109BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein CrcB homolog 2 (crcB2), partial CSB-YP769110BQE1
CSB-EP769110BQE1
CSB-BP769110BQE1
CSB-MP769110BQE1
CSB-EP769110BQE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Membrane protein insertase YidC 1 (yidC1), partial CSB-YP769111BQE1
CSB-EP769111BQE1
CSB-BP769111BQE1
CSB-MP769111BQE1
CSB-EP769111BQE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable cytosol aminopeptidase (pepA) CSB-YP769112BQE
CSB-EP769112BQE
CSB-BP769112BQE
CSB-MP769112BQE
CSB-EP769112BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holin-like protein CidA 1 (cidA1), partial CSB-YP769113BQE1
CSB-EP769113BQE1
CSB-BP769113BQE1
CSB-MP769113BQE1
CSB-EP769113BQE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Small, acid-soluble spore protein tlp (tlp) CSB-YP769114BQE
CSB-EP769114BQE
CSB-BP769114BQE
CSB-MP769114BQE
CSB-EP769114BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable transcriptional regulatory protein BA_0541/GBAA_0541/BAS0510 (BA_0541, GBAA_0541, BAS0510) CSB-YP769115BQE
CSB-EP769115BQE
CSB-BP769115BQE
CSB-MP769115BQE
CSB-EP769115BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Processive diacylglycerol glucosyltransferase (ugtP) CSB-YP769116BQE
CSB-EP769116BQE
CSB-BP769116BQE
CSB-MP769116BQE
CSB-EP769116BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin--NADP reductase 1 (BA_0352, GBAA_0352, BAS0337) CSB-YP769117BQE
CSB-EP769117BQE
CSB-BP769117BQE
CSB-MP769117BQE
CSB-EP769117BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 1-pyrroline-5-carboxylate dehydrogenase (rocA), partial CSB-YP769118BQE
CSB-EP769118BQE
CSB-BP769118BQE
CSB-MP769118BQE
CSB-EP769118BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP769119BQE
CSB-EP769119BQE
CSB-BP769119BQE
CSB-MP769119BQE
CSB-EP769119BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Demethylmenaquinone methyltransferase (ubiE) CSB-YP769120SNN
CSB-EP769120SNN
CSB-BP769120SNN
CSB-MP769120SNN
CSB-EP769120SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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