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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Helicoverpa armigera stunt virus Capsid protein (p71), partial CSB-YP769161HCAM
CSB-EP769161HCAM
CSB-BP769161HCAM
CSB-MP769161HCAM
CSB-EP769161HCAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Proline--tRNA ligase (proS), partial CSB-YP769162DSL
CSB-EP769162DSL
CSB-BP769162DSL
CSB-MP769162DSL
CSB-EP769162DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Lipoprotein signal peptidase (lspA), partial CSB-YP769163DSL1
CSB-EP769163DSL1
CSB-BP769163DSL1
CSB-MP769163DSL1
CSB-EP769163DSL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP769164DSL
CSB-EP769164DSL
CSB-BP769164DSL
CSB-MP769164DSL
CSB-EP769164DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Probable transcriptional regulatory protein CCA_00169 (CCA_00169) CSB-YP769165DSL
CSB-EP769165DSL
CSB-BP769165DSL
CSB-MP769165DSL
CSB-EP769165DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP769166DSL
CSB-EP769166DSL
CSB-BP769166DSL
CSB-MP769166DSL
CSB-EP769166DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae 30S ribosomal protein S5 (rpsE) CSB-YP769167DSL
CSB-EP769167DSL
CSB-BP769167DSL
CSB-MP769167DSL
CSB-EP769167DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae 30S ribosomal protein S3 (rpsC) CSB-YP769168DSL
CSB-EP769168DSL
CSB-BP769168DSL
CSB-MP769168DSL
CSB-EP769168DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP769169DSL
CSB-EP769169DSL
CSB-BP769169DSL
CSB-MP769169DSL
CSB-EP769169DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Ribosome-recycling factor (frr) CSB-YP769170DSL
CSB-EP769170DSL
CSB-BP769170DSL
CSB-MP769170DSL
CSB-EP769170DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Na (+)-translocating NADH-quinone reductase subunit A (nqrA) CSB-YP769171DSL
CSB-EP769171DSL
CSB-BP769171DSL
CSB-MP769171DSL
CSB-EP769171DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian infectious bursal disease virus RNA-directed RNA polymerase (VP1), partial CSB-YP769172AGAO
CSB-EP769172AGAO
CSB-BP769172AGAO
CSB-MP769172AGAO
CSB-EP769172AGAO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Infectious hematopoietic necrosis virus Nucleoprotein (N) CSB-YP769173ICAJ
CSB-EP769173ICAJ
CSB-BP769173ICAJ
CSB-MP769173ICAJ
CSB-EP769173ICAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alpha-1,3-galactosidase A (glaA), partial CSB-YP769174SNN
CSB-EP769174SNN
CSB-BP769174SNN
CSB-MP769174SNN
CSB-EP769174SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycerol kinase 3 (glpK3), partial CSB-YP769175SNN
CSB-EP769175SNN
CSB-BP769175SNN
CSB-MP769175SNN
CSB-EP769175SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Biotin synthase (bioB) CSB-YP769176SNN
CSB-EP769176SNN
CSB-BP769176SNN
CSB-MP769176SNN
CSB-EP769176SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Infectious hematopoietic necrosis virus Non-virion protein (NV) CSB-YP769177IAP
CSB-EP769177IAP
CSB-BP769177IAP
CSB-MP769177IAP
CSB-EP769177IAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP phosphoribosyltransferase (hisG) CSB-YP769178SNN
CSB-EP769178SNN
CSB-BP769178SNN
CSB-MP769178SNN
CSB-EP769178SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guanylate kinase (gmk) CSB-YP769179SNN
CSB-EP769179SNN
CSB-BP769179SNN
CSB-MP769179SNN
CSB-EP769179SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shikimate kinase (aroK) CSB-YP769180SNN
CSB-EP769180SNN
CSB-BP769180SNN
CSB-MP769180SNN
CSB-EP769180SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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