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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus Histidine--tRNA ligase 1 (hisS-1) CSB-YP770574BAM
CSB-EP770574BAM
CSB-BP770574BAM
CSB-MP770574BAM
CSB-EP770574BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP770575BAM
CSB-EP770575BAM
CSB-BP770575BAM
CSB-MP770575BAM
CSB-EP770575BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP-dependent Clp protease proteolytic subunit 1 (clpP1) CSB-YP770576BAM
CSB-EP770576BAM
CSB-BP770576BAM
CSB-MP770576BAM
CSB-EP770576BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus D-alanine--D-alanine ligase A (ddlA) CSB-YP770577BAM
CSB-EP770577BAM
CSB-BP770577BAM
CSB-MP770577BAM
CSB-EP770577BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus FMN-dependent NADH-azoreductase 2 (azoR2) CSB-YP770578BAM
CSB-EP770578BAM
CSB-BP770578BAM
CSB-MP770578BAM
CSB-EP770578BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Formate--tetrahydrofolate ligase (fhs), partial CSB-YP770579BAM
CSB-EP770579BAM
CSB-BP770579BAM
CSB-MP770579BAM
CSB-EP770579BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0145 protein BC_1816 (BC_1816) CSB-YP770580BAM
CSB-EP770580BAM
CSB-BP770580BAM
CSB-MP770580BAM
CSB-EP770580BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3-oxoacyl-[acyl-carrier-protein] synthase 3 protein 2 (fabH2) CSB-YP770581BAM
CSB-EP770581BAM
CSB-BP770581BAM
CSB-MP770581BAM
CSB-EP770581BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Pantothenate synthetase (panC) CSB-YP770582BAM
CSB-EP770582BAM
CSB-BP770582BAM
CSB-MP770582BAM
CSB-EP770582BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Chorismate synthase 1 (aroC1) CSB-YP770583BAM
CSB-EP770583BAM
CSB-BP770583BAM
CSB-MP770583BAM
CSB-EP770583BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Adapter protein mecA 2 (mecA2) CSB-YP770584BAM
CSB-EP770584BAM
CSB-BP770584BAM
CSB-MP770584BAM
CSB-EP770584BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP770585BAM
CSB-EP770585BAM
CSB-BP770585BAM
CSB-MP770585BAM
CSB-EP770585BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP phosphoribosyltransferase (hisG) CSB-YP770586BAM
CSB-EP770586BAM
CSB-BP770586BAM
CSB-MP770586BAM
CSB-EP770586BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Undecaprenyl-diphosphatase 2 (uppP2), partial CSB-YP770587BAM
CSB-EP770587BAM
CSB-BP770587BAM
CSB-MP770587BAM
CSB-EP770587BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphonoacetaldehyde hydrolase (phnX) CSB-YP770588BAM
CSB-EP770588BAM
CSB-BP770588BAM
CSB-MP770588BAM
CSB-EP770588BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Indole-3-glycerol phosphate synthase (trpC) CSB-YP770589BAM
CSB-EP770589BAM
CSB-BP770589BAM
CSB-MP770589BAM
CSB-EP770589BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable inorganic polyphosphate/ATP-NAD kinase 1 (ppnK1) CSB-YP770590BAM
CSB-EP770590BAM
CSB-BP770590BAM
CSB-MP770590BAM
CSB-EP770590BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3-oxoacyl-[acyl-carrier-protein] synthase 3 protein 1 (fabH1) CSB-YP770591BAM
CSB-EP770591BAM
CSB-BP770591BAM
CSB-MP770591BAM
CSB-EP770591BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP-dependent helicase/deoxyribonuclease subunit B (addB), partial CSB-YP770592BAM
CSB-EP770592BAM
CSB-BP770592BAM
CSB-MP770592BAM
CSB-EP770592BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glycerol kinase (glpK) CSB-YP770593BAM
CSB-EP770593BAM
CSB-BP770593BAM
CSB-MP770593BAM
CSB-EP770593BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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