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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus tRNA modification GTPase MnmE (mnmE) CSB-YP770534BAM
CSB-EP770534BAM
CSB-BP770534BAM
CSB-MP770534BAM
CSB-EP770534BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S18 (rpsR) CSB-YP770535BAM
CSB-EP770535BAM
CSB-BP770535BAM
CSB-MP770535BAM
CSB-EP770535BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Antiholin-like protein LrgA (lrgA), partial CSB-YP770536BAM1
CSB-EP770536BAM1
CSB-BP770536BAM1
CSB-MP770536BAM1
CSB-EP770536BAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Spermidine synthase 1 (speE1) CSB-YP770537BAM
CSB-EP770537BAM
CSB-BP770537BAM
CSB-MP770537BAM
CSB-EP770537BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Release factor glutamine methyltransferase (prmC) CSB-YP770538BAM
CSB-EP770538BAM
CSB-BP770538BAM
CSB-MP770538BAM
CSB-EP770538BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP synthase subunit c (atpE), partial CSB-YP770539BAM1
CSB-EP770539BAM1
CSB-BP770539BAM1
CSB-MP770539BAM1
CSB-EP770539BAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP synthase epsilon chain (atpC) CSB-YP770540BAM
CSB-EP770540BAM
CSB-BP770540BAM
CSB-MP770540BAM
CSB-EP770540BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase (fabZ) CSB-YP770541BAM
CSB-EP770541BAM
CSB-BP770541BAM
CSB-MP770541BAM
CSB-EP770541BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0145 protein BC_5181 (BC_5181) CSB-YP770542BAM
CSB-EP770542BAM
CSB-BP770542BAM
CSB-MP770542BAM
CSB-EP770542BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP770543BAM
CSB-EP770543BAM
CSB-BP770543BAM
CSB-MP770543BAM
CSB-EP770543BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methionine--tRNA ligase 2 (metG2), partial CSB-YP770544BAM
CSB-EP770544BAM
CSB-BP770544BAM
CSB-MP770544BAM
CSB-EP770544BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glucose-6-phosphate isomerase (pgi) CSB-YP770545BAM
CSB-EP770545BAM
CSB-BP770545BAM
CSB-MP770545BAM
CSB-EP770545BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Molybdenum cofactor biosynthesis protein B (moaB) CSB-YP770546BAM
CSB-EP770546BAM
CSB-BP770546BAM
CSB-MP770546BAM
CSB-EP770546BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Tyrosine--tRNA ligase 1 (tyrS1) CSB-YP770547BAM
CSB-EP770547BAM
CSB-BP770547BAM
CSB-MP770547BAM
CSB-EP770547BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Argininosuccinate synthase (argG) CSB-YP770548BAM
CSB-EP770548BAM
CSB-BP770548BAM
CSB-MP770548BAM
CSB-EP770548BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Dephospho-CoA kinase (coaE) CSB-YP770549BAM
CSB-EP770549BAM
CSB-BP770549BAM
CSB-MP770549BAM
CSB-EP770549BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP770550BAM
CSB-EP770550BAM
CSB-BP770550BAM
CSB-MP770550BAM
CSB-EP770550BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Porphobilinogen deaminase (hemC) CSB-YP770551BAM
CSB-EP770551BAM
CSB-BP770551BAM
CSB-MP770551BAM
CSB-EP770551BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus GTPase obg (obg) CSB-YP770552BAM
CSB-EP770552BAM
CSB-BP770552BAM
CSB-MP770552BAM
CSB-EP770552BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus D-tyrosyl-tRNA (Tyr) deacylase (dtd) CSB-YP770553BAM
CSB-EP770553BAM
CSB-BP770553BAM
CSB-MP770553BAM
CSB-EP770553BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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