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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Elongation factor 4 (lepA), partial CSB-YP770634BQE
CSB-EP770634BQE
CSB-BP770634BQE
CSB-MP770634BQE
CSB-EP770634BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S21 (rpsU) CSB-YP770635BQE
CSB-EP770635BQE
CSB-BP770635BQE
CSB-MP770635BQE
CSB-EP770635BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP770636BQE
CSB-EP770636BQE
CSB-BP770636BQE
CSB-MP770636BQE
CSB-EP770636BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Octanoyltransferase LipM (lipM) CSB-YP770637BQE
CSB-EP770637BQE
CSB-BP770637BQE
CSB-MP770637BQE
CSB-EP770637BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP770638BQE
CSB-EP770638BQE
CSB-BP770638BQE
CSB-MP770638BQE
CSB-EP770638BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease Z (rnz) CSB-YP770639BQE
CSB-EP770639BQE
CSB-BP770639BQE
CSB-MP770639BQE
CSB-EP770639BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP770640BQE
CSB-EP770640BQE
CSB-BP770640BQE
CSB-MP770640BQE
CSB-EP770640BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Segregation and condensation protein A (scpA) CSB-YP770641BQE
CSB-EP770641BQE
CSB-BP770641BQE
CSB-MP770641BQE
CSB-EP770641BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX) CSB-YP770642BQE
CSB-EP770642BQE
CSB-BP770642BQE
CSB-MP770642BQE
CSB-EP770642BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide deformylase 2 (def2) CSB-YP770643BQE
CSB-EP770643BQE
CSB-BP770643BQE
CSB-MP770643BQE
CSB-EP770643BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Regulatory protein spx 2 (spxA2) CSB-YP770644BQE
CSB-EP770644BQE
CSB-BP770644BQE
CSB-MP770644BQE
CSB-EP770644BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidine--tRNA ligase 1 (hisS-1) CSB-YP770645BQE
CSB-EP770645BQE
CSB-BP770645BQE
CSB-MP770645BQE
CSB-EP770645BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0271 protein BA_3095/GBAA_3095/BAS2880 (BA_3095, GBAA_3095, BAS2880) CSB-YP770646BQE
CSB-EP770646BQE
CSB-BP770646BQE
CSB-MP770646BQE
CSB-EP770646BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidinol-phosphate aminotransferase 2 (hisC2) CSB-YP770647BQE
CSB-EP770647BQE
CSB-BP770647BQE
CSB-MP770647BQE
CSB-EP770647BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kynureninase (kynU) CSB-YP770648BQE
CSB-EP770648BQE
CSB-BP770648BQE
CSB-MP770648BQE
CSB-EP770648BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0397 protein BA_2640/GBAA_2640/BAS2460 (BA_2640, GBAA_2640, BAS2460), partial CSB-YP770649BQE1
CSB-EP770649BQE1
CSB-BP770649BQE1
CSB-MP770649BQE1
CSB-EP770649BQE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stage II sporulation protein SB (spoIISB) CSB-YP770650BQE
CSB-EP770650BQE
CSB-BP770650BQE
CSB-MP770650BQE
CSB-EP770650BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylmalonate semialdehyde dehydrogenase [acylating] 1 (iolA1) CSB-YP770651BQE
CSB-EP770651BQE
CSB-BP770651BQE
CSB-MP770651BQE
CSB-EP770651BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thymidylate synthase (thyA) CSB-YP770652BQE
CSB-EP770652BQE
CSB-BP770652BQE
CSB-MP770652BQE
CSB-EP770652BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NADPH dehydrogenase (namA) CSB-YP770653BQE
CSB-EP770653BQE
CSB-BP770653BQE
CSB-MP770653BQE
CSB-EP770653BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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