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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Dictyostelium discoideum Mitochondrial-processing peptidase subunit alpha-1 (mppA1), partial CSB-YP771295DKK
CSB-EP771295DKK
CSB-BP771295DKK
CSB-MP771295DKK
CSB-EP771295DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable T4-type lysozyme 2 (DDB_G0274291) CSB-YP771296DKK
CSB-EP771296DKK
CSB-BP771296DKK
CSB-MP771296DKK
CSB-EP771296DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable acetyl-CoA acetyltransferase (DDB_G0271544) CSB-YP771297DKK
CSB-EP771297DKK
CSB-BP771297DKK
CSB-MP771297DKK
CSB-EP771297DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable basic-leucine zipper transcription factor H (bzpH), partial CSB-YP771298DKK
CSB-EP771298DKK
CSB-BP771298DKK
CSB-MP771298DKK
CSB-EP771298DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable ribosome biogenesis protein RLP24 (rlp24) CSB-YP771299DKK
CSB-EP771299DKK
CSB-BP771299DKK
CSB-MP771299DKK
CSB-EP771299DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Modifier of mdg4 (mod (mdg4)), partial CSB-YP771300DLU
CSB-EP771300DLU
CSB-BP771300DLU
CSB-MP771300DLU
CSB-EP771300DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ascidia sydneiensis samea Vanadium-binding protein 2 (VANABIN2) CSB-YP771382AGAV
CSB-EP771382AGAV
CSB-BP771382AGAV
CSB-MP771382AGAV
CSB-EP771382AGAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma schmidti U5-theraphotoxin-Hh1a CSB-YP771383HUL
CSB-EP771383HUL
CSB-BP771383HUL
CSB-MP771383HUL
CSB-EP771383HUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0468 protein C16orf80 homolog (C54C6.6) CSB-YP771384CXY
CSB-EP771384CXY
CSB-BP771384CXY
CSB-MP771384CXY
CSB-EP771384CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Hsc70-interacting protein 2 (HIP-R) CSB-YP771385DLU
CSB-EP771385DLU
CSB-BP771385DLU
CSB-MP771385DLU
CSB-EP771385DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipoyl synthase, apicoplast (lipA) CSB-YP771386TOV
CSB-EP771386TOV
CSB-BP771386TOV
CSB-MP771386TOV
CSB-EP771386TOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable elongator complex protein 2 (elp2), partial CSB-YP771388DKK
CSB-EP771388DKK
CSB-BP771388DKK
CSB-MP771388DKK
CSB-EP771388DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0275587 (DDB_G0275587) CSB-YP771389DKK
CSB-EP771389DKK
CSB-BP771389DKK
CSB-MP771389DKK
CSB-EP771389DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0275685 (DDB_G0275685) CSB-YP771390DKK
CSB-EP771390DKK
CSB-BP771390DKK
CSB-MP771390DKK
CSB-EP771390DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum HssA/B-like protein DDB_G0295685 (DDB_G0295685) CSB-YP771391DKK
CSB-EP771391DKK
CSB-BP771391DKK
CSB-MP771391DKK
CSB-EP771391DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Oxysterol-binding protein 5 (osbE) CSB-YP771392DKK
CSB-EP771392DKK
CSB-BP771392DKK
CSB-MP771392DKK
CSB-EP771392DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Uncharacterized protein DDB_G0274935 (DDB_G0274935) CSB-YP771393DKK
CSB-EP771393DKK
CSB-BP771393DKK
CSB-MP771393DKK
CSB-EP771393DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Countin-3 (ctnC) CSB-YP771394DKK
CSB-EP771394DKK
CSB-BP771394DKK
CSB-MP771394DKK
CSB-EP771394DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0276421 (DDB_G0276421) CSB-YP771395DKK
CSB-EP771395DKK
CSB-BP771395DKK
CSB-MP771395DKK
CSB-EP771395DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum LIM domain-containing protein F (limF) CSB-YP771396DKK
CSB-EP771396DKK
CSB-BP771396DKK
CSB-MP771396DKK
CSB-EP771396DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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