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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Comamonas testosteroni 4-hydroxy-2-oxovalerate aldolase 2 (tesG) CSB-YP772792CNN
CSB-EP772792CNN
CSB-BP772792CNN
CSB-MP772792CNN
CSB-EP772792CNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine/sarcosine N-methyltransferase CSB-YP772793DNJ
CSB-EP772793DNJ
CSB-BP772793DNJ
CSB-MP772793DNJ
CSB-EP772793DNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative phosphotransferase in pntA-hypC2 intergenic region CSB-YP772794TEB
CSB-EP772794TEB
CSB-BP772794TEB
CSB-MP772794TEB
CSB-EP772794TEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Response regulator saeR (saeR) CSB-YP772795FLG
CSB-EP772795FLG
CSB-BP772795FLG
CSB-MP772795FLG
CSB-EP772795FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porcine adenovirus A serotype 3 Penton protein (PIII) CSB-YP772796PIAK
CSB-EP772796PIAK
CSB-BP772796PIAK
CSB-MP772796PIAK
CSB-EP772796PIAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human papillomavirus type 6a protein E4 (E4) CSB-YP772797HOM
CSB-BP772797HOM
CSB-MP772797HOM
CSB-EP772797HOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paramecium bursaria Chlorella virus 1 Probable DNA polymerase sliding clamp 1 (A193L) CSB-YP772798ERU
CSB-EP772798ERU
CSB-BP772798ERU
CSB-MP772798ERU
CSB-EP772798ERU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP772799BPS
CSB-EP772799BPS
CSB-BP772799BPS
CSB-MP772799BPS
CSB-EP772799BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peanut clump virus Suppressor of RNA silencing (ORF2) CSB-YP772800PAAX
CSB-EP772800PAAX
CSB-BP772800PAAX
CSB-MP772800PAAX
CSB-EP772800PAAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S21 (rpsU) CSB-YP772801ADAK
CSB-EP772801ADAK
CSB-BP772801ADAK
CSB-MP772801ADAK
CSB-EP772801ADAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Capsular polysaccharide phosphotransferase lcbA (lcbA) CSB-YP772802AXW
CSB-EP772802AXW
CSB-BP772802AXW
CSB-MP772802AXW
CSB-EP772802AXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Probable efflux pump periplasmic linker protein sepA (sepA) CSB-YP772803PZN
CSB-EP772803PZN
CSB-BP772803PZN
CSB-MP772803PZN
CSB-EP772803PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Capsular polysaccharide phosphotransferase lcbA (lcbA) CSB-YP772804AXW
CSB-EP772804AXW
CSB-BP772804AXW
CSB-MP772804AXW
CSB-EP772804AXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus acidophilus Chaperone protein DnaJ (dnaJ) CSB-YP772805LME
CSB-EP772805LME
CSB-BP772805LME
CSB-MP772805LME
CSB-EP772805LME-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Capsular polysaccharide phosphotransferase CSB-YP772806ACR
CSB-EP772806ACR
CSB-BP772806ACR
CSB-MP772806ACR
CSB-EP772806ACR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-hydroxyphenylacetate decarboxylase large subunit (hpdB), partial CSB-YP772807DUN
CSB-EP772807DUN
CSB-BP772807DUN
CSB-MP772807DUN
CSB-EP772807DUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chorismate mutase AroH (aroH) CSB-YP772808TNR
CSB-EP772808TNR
CSB-BP772808TNR
CSB-MP772808TNR
CSB-EP772808TNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tryptophan synthase beta chain (trpB) CSB-YP772809NFZ
CSB-EP772809NFZ
CSB-BP772809NFZ
CSB-MP772809NFZ
CSB-EP772809NFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas fluorescens Kynurenine 3-monooxygenase (kmo) CSB-YP772810FFT
CSB-EP772810FFT
CSB-BP772810FFT
CSB-MP772810FFT
CSB-EP772810FFT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidinol-phosphate aminotransferase (hisC) CSB-YP772811BXF
CSB-EP772811BXF
CSB-BP772811BXF
CSB-MP772811BXF
CSB-EP772811BXF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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