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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0271918 (DDB_G0271918) CSB-YP771397DKK
CSB-EP771397DKK
CSB-BP771397DKK
CSB-MP771397DKK
CSB-EP771397DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable caffeoyl-CoA O-methyltransferase 2 (omt6) CSB-YP771398DKK
CSB-EP771398DKK
CSB-BP771398DKK
CSB-MP771398DKK
CSB-EP771398DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Transmembrane protein DDB_G0272716 (DDB_G0272716), partial CSB-YP771399DKK1
CSB-EP771399DKK1
CSB-BP771399DKK1
CSB-MP771399DKK1
CSB-EP771399DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Countin-1 (ctnA) CSB-YP771400DKK
CSB-EP771400DKK
CSB-BP771400DKK
CSB-MP771400DKK
CSB-EP771400DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Transmembrane protein DDB_G0274347 (DDB_G0274347), partial CSB-YP771401DKK1
CSB-EP771401DKK1
CSB-BP771401DKK1
CSB-MP771401DKK1
CSB-EP771401DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum UPF0519 protein A (sigN137) CSB-YP771402DKK
CSB-EP771402DKK
CSB-BP771402DKK
CSB-MP771402DKK
CSB-EP771402DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Histone acetyltransferase type B catalytic subunit DDB_G0274269 (DDB_G0274269) CSB-YP771403DKK
CSB-EP771403DKK
CSB-BP771403DKK
CSB-MP771403DKK
CSB-EP771403DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0277003 (DDB_G0277003) CSB-YP771404DKK
CSB-EP771404DKK
CSB-BP771404DKK
CSB-MP771404DKK
CSB-EP771404DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0271554 (DDB_G0271554) CSB-YP771405DKK
CSB-EP771405DKK
CSB-BP771405DKK
CSB-MP771405DKK
CSB-EP771405DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum DNA-directed RNA polymerase II subunit rpb11 (polr2j) CSB-YP771406DKK
CSB-EP771406DKK
CSB-BP771406DKK
CSB-MP771406DKK
CSB-EP771406DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0271974 (DDB_G0271974) CSB-YP771407DKK
CSB-EP771407DKK
CSB-BP771407DKK
CSB-MP771407DKK
CSB-EP771407DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum HssA/B-like protein 28 (hssl28) CSB-YP771408DKK
CSB-EP771408DKK
CSB-BP771408DKK
CSB-MP771408DKK
CSB-EP771408DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Trafficking protein particle complex subunit 3 (trappc3) CSB-YP771409DKK
CSB-EP771409DKK
CSB-BP771409DKK
CSB-MP771409DKK
CSB-EP771409DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Cullin-associated NEDD8-dissociated protein 1 (cand1), partial CSB-YP771410DKK
CSB-EP771410DKK
CSB-BP771410DKK
CSB-MP771410DKK
CSB-EP771410DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum AP-2 complex subunit alpha-2 (ap2a1-1), partial CSB-YP771411DKK
CSB-EP771411DKK
CSB-BP771411DKK
CSB-MP771411DKK
CSB-EP771411DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum DNA cross-link repair 1 protein (dclre1), partial CSB-YP771412DKK
CSB-EP771412DKK
CSB-BP771412DKK
CSB-MP771412DKK
CSB-EP771412DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Ribosome maturation protein SBDS (sbds) CSB-YP771413DKK
CSB-EP771413DKK
CSB-BP771413DKK
CSB-MP771413DKK
CSB-EP771413DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Eukaryotic translation initiation factor 3 subunit L (eif3L), partial CSB-YP771414DKK
CSB-EP771414DKK
CSB-BP771414DKK
CSB-MP771414DKK
CSB-EP771414DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aplysia californica Protein PRQFV-amide CSB-YP771415DNM
CSB-EP771415DNM
CSB-BP771415DNM
CSB-MP771415DNM
CSB-EP771415DNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Culex pipiens pipiens Cecropin-B1 (CECB1) CSB-YP771416CUL
CSB-EP771416CUL
CSB-BP771416CUL
CSB-MP771416CUL
CSB-EP771416CUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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