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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oryza sativa subsp. japonica Putative alpha-L-fucosidase 1 (Os04g0560400, LOC_Os04g47280) CSB-YP773531OFG
CSB-EP773531OFG
CSB-BP773531OFG
CSB-MP773531OFG
CSB-EP773531OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative lipoxygenase 5 (Os04g0447100, LOC_Os04g37430), partial CSB-YP773532OFG
CSB-EP773532OFG
CSB-BP773532OFG
CSB-MP773532OFG
CSB-EP773532OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable protein transport Sec1a (Os04g0252400, LOC_Os04g18030), partial CSB-YP773533OFG
CSB-EP773533OFG
CSB-BP773533OFG
CSB-MP773533OFG
CSB-EP773533OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artemisia spiciformis Monoterpene synthase FDS-5, chloroplastic (FDS-5) CSB-YP773534AGAL
CSB-EP773534AGAL
CSB-BP773534AGAL
CSB-MP773534AGAL
CSB-EP773534AGAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petroselinum crispum Flavone synthase (FNSI) CSB-YP773535PGL
CSB-EP773535PGL
CSB-BP773535PGL
CSB-MP773535PGL
CSB-EP773535PGL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Thioredoxin-like protein HCF164, chloroplastic (Os03g0767500, LOC_Os03g55820) CSB-YP773536OFG
CSB-EP773536OFG
CSB-BP773536OFG
CSB-MP773536OFG
CSB-EP773536OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable protein phosphatase 2C 36 (Os03g0832400, LOC_Os03g61690) CSB-YP773537OFG
CSB-EP773537OFG
CSB-BP773537OFG
CSB-MP773537OFG
CSB-EP773537OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cytochrome P450 78A11 (CYP78A11), partial CSB-YP773538OFG1
CSB-EP773538OFG1
CSB-BP773538OFG1
CSB-MP773538OFG1
CSB-EP773538OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana UDP-glycosyltransferase 73B4 (UGT73B4) CSB-YP773539DOA
CSB-EP773539DOA
CSB-BP773539DOA
CSB-MP773539DOA
CSB-EP773539DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apomys insignis Cytochrome b (MT-CYB), partial CSB-YP773540AGAZ
CSB-EP773540AGAZ
CSB-BP773540AGAZ
CSB-MP773540AGAZ
CSB-EP773540AGAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hemiechinus auritus Cytochrome b (MT-CYB), partial CSB-YP773541HEAD
CSB-EP773541HEAD
CSB-BP773541HEAD
CSB-MP773541HEAD
CSB-EP773541HEAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Myotis frater Cytochrome b (MT-CYB), partial CSB-YP773542MHAC
CSB-EP773542MHAC
CSB-BP773542MHAC
CSB-MP773542MHAC
CSB-EP773542MHAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Myotis vivesi Cytochrome b (MT-CYB), partial CSB-YP773543MHAD
CSB-EP773543MHAD
CSB-BP773543MHAD
CSB-MP773543MHAD
CSB-EP773543MHAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Platalina genovensium Cytochrome b (MT-CYB), partial CSB-YP773544PIAQ
CSB-EP773544PIAQ
CSB-BP773544PIAQ
CSB-MP773544PIAQ
CSB-EP773544PIAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Cytochrome c oxidase subunit 2 (COX2), partial CSB-YP773545DOT1
CSB-EP773545DOT1
CSB-BP773545DOT1
CSB-MP773545DOT1
CSB-EP773545DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Calycanthus floridus var. glaucus NAD (P)H-quinone oxidoreductase subunit 1, chloroplastic (ndhA), partial CSB-YP773546CBM1
CSB-EP773546CBM1
CSB-BP773546CBM1
CSB-MP773546CBM1
CSB-EP773546CBM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Calycanthus floridus var. glaucus 30S ribosomal protein S19, chloroplastic (rps19) CSB-YP773547CBM
CSB-EP773547CBM
CSB-BP773547CBM
CSB-MP773547CBM
CSB-EP773547CBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Calycanthus floridus var. glaucus 30S ribosomal protein S11, chloroplastic (rps11) CSB-YP773548CBM
CSB-EP773548CBM
CSB-BP773548CBM
CSB-MP773548CBM
CSB-EP773548CBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Calycanthus floridus var. glaucus Photosystem I reaction center subunit IX (psaJ), partial CSB-YP773549CBM
CSB-EP773549CBM
CSB-BP773549CBM
CSB-MP773549CBM
CSB-EP773549CBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Calycanthus floridus var. glaucus Photosystem I reaction center subunit VIII (psaI), partial CSB-YP773550CBM
CSB-EP773550CBM
CSB-BP773550CBM
CSB-MP773550CBM
CSB-EP773550CBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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