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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Dictyostelium discoideum Ras guanine nucleotide exchange factor Q (gefQ), partial CSB-YP774752DKK
CSB-EP774752DKK
CSB-BP774752DKK
CSB-MP774752DKK
CSB-EP774752DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Superoxide-generating NADPH oxidase heavy chain subunit B (noxB), partial CSB-YP774753DKK1
CSB-EP774753DKK1
CSB-BP774753DKK1
CSB-MP774753DKK1
CSB-EP774753DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Alanine--tRNA ligase, mitochondrial (malaS), partial CSB-YP774754DKK
CSB-EP774754DKK
CSB-BP774754DKK
CSB-MP774754DKK
CSB-EP774754DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0275597 (DDB_G0275597) CSB-YP774755DKK
CSB-EP774755DKK
CSB-BP774755DKK
CSB-MP774755DKK
CSB-EP774755DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Uncharacterized protein DDB_G0276477 (DDB_G0276477) CSB-YP774756DKK
CSB-EP774756DKK
CSB-BP774756DKK
CSB-MP774756DKK
CSB-EP774756DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Mannose-1-phosphate guanyltransferase alpha (gmppA) CSB-YP774757DKK
CSB-EP774757DKK
CSB-BP774757DKK
CSB-MP774757DKK
CSB-EP774757DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Hydroxymethylglutaryl-CoA synthase B (hgsB) CSB-YP774758DKK
CSB-EP774758DKK
CSB-BP774758DKK
CSB-MP774758DKK
CSB-EP774758DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum ABC transporter G family member 8 (abcG8), partial CSB-YP774759DKK1
CSB-EP774759DKK1
CSB-BP774759DKK1
CSB-MP774759DKK1
CSB-EP774759DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Uncharacterized protein DDB_G0276467 (DDB_G0276467) CSB-YP774760DKK
CSB-EP774760DKK
CSB-BP774760DKK
CSB-MP774760DKK
CSB-EP774760DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (pdhB) CSB-YP774761DKK
CSB-EP774761DKK
CSB-BP774761DKK
CSB-MP774761DKK
CSB-EP774761DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Small heat shock protein hspG1 (hspG1) CSB-YP774762DKK
CSB-EP774762DKK
CSB-BP774762DKK
CSB-MP774762DKK
CSB-EP774762DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Mitochondrial substrate carrier family protein I (mcfI), partial CSB-YP774763DKK1
CSB-EP774763DKK1
CSB-BP774763DKK1
CSB-MP774763DKK1
CSB-EP774763DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Protein disulfide-isomerase 1 (pdi1) CSB-YP774764DKK
CSB-EP774764DKK
CSB-BP774764DKK
CSB-MP774764DKK
CSB-EP774764DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0275629 (DDB_G0275629) CSB-YP774765DKK
CSB-EP774765DKK
CSB-BP774765DKK
CSB-MP774765DKK
CSB-EP774765DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Uncharacterized protein DDB_G0272718 (DDB_G0272718) CSB-YP774766DKK
CSB-EP774766DKK
CSB-BP774766DKK
CSB-MP774766DKK
CSB-EP774766DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0272194 (DDB_G0272194) CSB-YP774767DKK
CSB-EP774767DKK
CSB-BP774767DKK
CSB-MP774767DKK
CSB-EP774767DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum COMM domain-containing protein 5 (commd5) CSB-YP774768DKK
CSB-EP774768DKK
CSB-BP774768DKK
CSB-MP774768DKK
CSB-EP774768DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (ndufa13), partial CSB-YP774769DKK1
CSB-EP774769DKK1
CSB-BP774769DKK1
CSB-MP774769DKK1
CSB-EP774769DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0274271 (DDB_G0274271) CSB-YP774770DKK
CSB-EP774770DKK
CSB-BP774770DKK
CSB-MP774770DKK
CSB-EP774770DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum 26S protease regulatory subunit 7 (psmC2) CSB-YP774771DKK
CSB-EP774771DKK
CSB-BP774771DKK
CSB-MP774771DKK
CSB-EP774771DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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