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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Pseudomonas putida Ribosomal RNA small subunit methyltransferase B (rsmB) CSB-YP773432FGB
CSB-EP773432FGB
CSB-BP773432FGB
CSB-MP773432FGB
CSB-EP773432FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP773433LMS
CSB-EP773433LMS
CSB-BP773433LMS
CSB-MP773433LMS
CSB-EP773433LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Rhamnulokinase (rhaB) CSB-YP773434LMS
CSB-EP773434LMS
CSB-BP773434LMS
CSB-MP773434LMS
CSB-EP773434LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Probable transaldolase (tal) CSB-YP773435LMS
CSB-EP773435LMS
CSB-BP773435LMS
CSB-MP773435LMS
CSB-EP773435LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Adenine deaminase (ade), partial CSB-YP773436LMS
CSB-EP773436LMS
CSB-BP773436LMS
CSB-MP773436LMS
CSB-EP773436LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Elongation factor 4 2 (lepA2), partial CSB-YP773437LMS
CSB-EP773437LMS
CSB-BP773437LMS
CSB-MP773437LMS
CSB-EP773437LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP773438LMS
CSB-EP773438LMS
CSB-BP773438LMS
CSB-MP773438LMS
CSB-EP773438LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP773439LMS
CSB-EP773439LMS
CSB-BP773439LMS
CSB-MP773439LMS
CSB-EP773439LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP773440LMS
CSB-EP773440LMS
CSB-BP773440LMS
CSB-MP773440LMS
CSB-EP773440LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Putative sugar uptake protein lp_2503 (lp_2503), partial CSB-YP773441LMS
CSB-EP773441LMS
CSB-BP773441LMS
CSB-MP773441LMS
CSB-EP773441LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum ATP synthase subunit c (atpE), partial CSB-YP773442LMS1
CSB-EP773442LMS1
CSB-BP773442LMS1
CSB-MP773442LMS1
CSB-EP773442LMS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum ATP synthase epsilon chain (atpC) CSB-YP773443LMS
CSB-EP773443LMS
CSB-BP773443LMS
CSB-MP773443LMS
CSB-EP773443LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Septation ring formation regulator EzrA (ezrA), partial CSB-YP773444LMS
CSB-EP773444LMS
CSB-BP773444LMS
CSB-MP773444LMS
CSB-EP773444LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Queuine tRNA-ribosyltransferase (tgt) CSB-YP773445LMS
CSB-EP773445LMS
CSB-BP773445LMS
CSB-MP773445LMS
CSB-EP773445LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Glutamate racemase (murI) CSB-YP773446LMS
CSB-EP773446LMS
CSB-BP773446LMS
CSB-MP773446LMS
CSB-EP773446LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Regulatory protein spx (spxA) CSB-YP773447LMS
CSB-EP773447LMS
CSB-BP773447LMS
CSB-MP773447LMS
CSB-EP773447LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP773448LMS
CSB-EP773448LMS
CSB-BP773448LMS
CSB-MP773448LMS
CSB-EP773448LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Isoleucine--tRNA ligase (ileS), partial CSB-YP773449LMS
CSB-EP773449LMS
CSB-BP773449LMS
CSB-MP773449LMS
CSB-EP773449LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Phosphopantetheine adenylyltransferase (coaD) CSB-YP773450LMS
CSB-EP773450LMS
CSB-BP773450LMS
CSB-MP773450LMS
CSB-EP773450LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum GTPase obg (obg) CSB-YP773451LMS
CSB-EP773451LMS
CSB-BP773451LMS
CSB-MP773451LMS
CSB-EP773451LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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