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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus 50S ribosomal protein L32 (rpmF) CSB-YP774011BAM
CSB-EP774011BAM
CSB-BP774011BAM
CSB-MP774011BAM
CSB-EP774011BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP774012BAM
CSB-EP774012BAM
CSB-BP774012BAM
CSB-MP774012BAM
CSB-EP774012BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Carbamoyl-phosphate synthase small chain (carA) CSB-YP774013BAM
CSB-EP774013BAM
CSB-BP774013BAM
CSB-MP774013BAM
CSB-EP774013BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP774014BAM
CSB-EP774014BAM
CSB-BP774014BAM
CSB-MP774014BAM
CSB-EP774014BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribonuclease 3 (rnc) CSB-YP774015BAM
CSB-EP774015BAM
CSB-BP774015BAM
CSB-MP774015BAM
CSB-EP774015BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribosome biogenesis GTPase A (rbgA) CSB-YP774016BAM
CSB-EP774016BAM
CSB-BP774016BAM
CSB-MP774016BAM
CSB-EP774016BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus GTP-sensing transcriptional pleiotropic repressor CodY (codY) CSB-YP774017BAM
CSB-EP774017BAM
CSB-BP774017BAM
CSB-MP774017BAM
CSB-EP774017BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA translocase FtsK (ftsK), partial CSB-YP774018BAM1
CSB-EP774018BAM1
CSB-BP774018BAM1
CSB-MP774018BAM1
CSB-EP774018BAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA mismatch repair protein mutL (mutL), partial CSB-YP774019BAM
CSB-EP774019BAM
CSB-BP774019BAM
CSB-MP774019BAM
CSB-EP774019BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Holin-like protein CidA 1 (cidA1), partial CSB-YP774020BAM1
CSB-EP774020BAM1
CSB-BP774020BAM1
CSB-MP774020BAM1
CSB-EP774020BAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0303 protein BC_3395 (BC_3395) CSB-YP774021BAM
CSB-EP774021BAM
CSB-BP774021BAM
CSB-MP774021BAM
CSB-EP774021BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Protein ArsC (arsC) CSB-YP774022BAM
CSB-EP774022BAM
CSB-BP774022BAM
CSB-MP774022BAM
CSB-EP774022BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0178 protein BC_3040 (BC_3040) CSB-YP774023BAM
CSB-EP774023BAM
CSB-BP774023BAM
CSB-MP774023BAM
CSB-EP774023BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Aliphatic sulfonates import ATP-binding protein SsuB (ssuB) CSB-YP774024BAM
CSB-EP774024BAM
CSB-BP774024BAM
CSB-MP774024BAM
CSB-EP774024BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Undecaprenyl-diphosphatase 3 (uppP3), partial CSB-YP774025BAM
CSB-EP774025BAM
CSB-BP774025BAM
CSB-MP774025BAM
CSB-EP774025BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP774026BAM
CSB-EP774026BAM
CSB-BP774026BAM
CSB-MP774026BAM
CSB-EP774026BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Thymidylate synthase (thyA) CSB-YP774027BAM
CSB-EP774027BAM
CSB-BP774027BAM
CSB-MP774027BAM
CSB-EP774027BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Homoserine kinase (thrB) CSB-YP774028BAM
CSB-EP774028BAM
CSB-BP774028BAM
CSB-MP774028BAM
CSB-EP774028BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Dihydroxy-acid dehydratase (ilvD), partial CSB-YP774029BAM
CSB-EP774029BAM
CSB-BP774029BAM
CSB-MP774029BAM
CSB-EP774029BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Cell cycle protein GpsB (gpsB) CSB-YP774030BAM
CSB-EP774030BAM
CSB-BP774030BAM
CSB-MP774030BAM
CSB-EP774030BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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