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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant UPF0271 protein SAV_6939 (SAV_6939) CSB-YP774211SNN
CSB-EP774211SNN
CSB-BP774211SNN
CSB-MP774211SNN
CSB-EP774211SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carbamoyl-phosphate synthase small chain (carA) CSB-YP774212SNN
CSB-EP774212SNN
CSB-BP774212SNN
CSB-MP774212SNN
CSB-EP774212SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S4 (rpsD) CSB-YP774213SNN
CSB-EP774213SNN
CSB-BP774213SNN
CSB-MP774213SNN
CSB-EP774213SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Threonine--tRNA ligase (thrS), partial CSB-YP774214SNN
CSB-EP774214SNN
CSB-BP774214SNN
CSB-MP774214SNN
CSB-EP774214SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Jembrana disease virus Protein Tat (tat) CSB-YP774215JAAF
CSB-EP774215JAAF
CSB-BP774215JAAF
CSB-MP774215JAAF
CSB-EP774215JAAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP774216SNN
CSB-EP774216SNN
CSB-BP774216SNN
CSB-MP774216SNN
CSB-EP774216SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pup--protein ligase (pafA) CSB-YP774217SNN
CSB-EP774217SNN
CSB-BP774217SNN
CSB-MP774217SNN
CSB-EP774217SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (mshC) CSB-YP774218SNN
CSB-EP774218SNN
CSB-BP774218SNN
CSB-MP774218SNN
CSB-EP774218SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tyrosine--tRNA ligase (tyrS) CSB-YP774219SNN
CSB-EP774219SNN
CSB-BP774219SNN
CSB-MP774219SNN
CSB-EP774219SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protoheme IX farnesyltransferase (ctaB), partial CSB-YP774220SNN
CSB-EP774220SNN
CSB-BP774220SNN
CSB-MP774220SNN
CSB-EP774220SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-dehydroquinate dehydratase (aroQ) CSB-YP774221SNN
CSB-EP774221SNN
CSB-BP774221SNN
CSB-MP774221SNN
CSB-EP774221SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tryptophan synthase beta chain (trpB) CSB-YP774222SNN
CSB-EP774222SNN
CSB-BP774222SNN
CSB-MP774222SNN
CSB-EP774222SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidinol-phosphate aminotransferase (hisC) CSB-YP774223SNN
CSB-EP774223SNN
CSB-BP774223SNN
CSB-MP774223SNN
CSB-EP774223SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE), partial CSB-YP774224SNN
CSB-EP774224SNN
CSB-BP774224SNN
CSB-MP774224SNN
CSB-EP774224SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable cytochrome c oxidase polypeptide 4 (ctaF), partial CSB-YP774225SNN1
CSB-EP774225SNN1
CSB-BP774225SNN1
CSB-MP774225SNN1
CSB-EP774225SNN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipoyl synthase (lipA) CSB-YP774226SNN
CSB-EP774226SNN
CSB-BP774226SNN
CSB-MP774226SNN
CSB-EP774226SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deoxyguanosinetriphosphate triphosphohydrolase-like protein (dgt) CSB-YP774227SNN
CSB-EP774227SNN
CSB-BP774227SNN
CSB-MP774227SNN
CSB-EP774227SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable 5-dehydro-4-deoxyglucarate dehydratase 2 (SAV_5580) CSB-YP774228SNN
CSB-EP774228SNN
CSB-BP774228SNN
CSB-MP774228SNN
CSB-EP774228SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate 5-kinase (proB) CSB-YP774229SNN
CSB-EP774229SNN
CSB-BP774229SNN
CSB-MP774229SNN
CSB-EP774229SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0256 protein SAV_5428 (SAV_5428) CSB-YP774230SNN
CSB-EP774230SNN
CSB-BP774230SNN
CSB-MP774230SNN
CSB-EP774230SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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