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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Heliothis zea nuclear polyhedrosis virus Very late expression factor 1 (VLF-1) CSB-YP774191HVG
CSB-EP774191HVG
CSB-BP774191HVG
CSB-MP774191HVG
CSB-EP774191HVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Putative 3-methyladenine DNA glycosylase (CCA_00239) CSB-YP774192DSL
CSB-EP774192DSL
CSB-BP774192DSL
CSB-MP774192DSL
CSB-EP774192DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Transcriptional repressor NrdR (nrdR) CSB-YP774193DSL
CSB-EP774193DSL
CSB-BP774193DSL
CSB-MP774193DSL
CSB-EP774193DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae 50S ribosomal protein L21 (rplU) CSB-YP774194DSL
CSB-EP774194DSL
CSB-BP774194DSL
CSB-MP774194DSL
CSB-EP774194DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Undecaprenyl pyrophosphate synthase (uppS) CSB-YP774195DSL
CSB-EP774195DSL
CSB-BP774195DSL
CSB-MP774195DSL
CSB-EP774195DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP774196DSL
CSB-EP774196DSL
CSB-BP774196DSL
CSB-MP774196DSL
CSB-EP774196DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP774197DSL
CSB-EP774197DSL
CSB-BP774197DSL
CSB-MP774197DSL
CSB-EP774197DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae 50S ribosomal protein L18 (rplR) CSB-YP774198DSL
CSB-EP774198DSL
CSB-BP774198DSL
CSB-MP774198DSL
CSB-EP774198DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae 30S ribosomal protein S17 (rpsQ) CSB-YP774199DSL
CSB-EP774199DSL
CSB-BP774199DSL
CSB-MP774199DSL
CSB-EP774199DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae 50S ribosomal protein L23 (rplW) CSB-YP774200DSL
CSB-EP774200DSL
CSB-BP774200DSL
CSB-MP774200DSL
CSB-EP774200DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Apolipoprotein N-acyltransferase (lnt), partial CSB-YP774201DSL1
CSB-EP774201DSL1
CSB-BP774201DSL1
CSB-MP774201DSL1
CSB-EP774201DSL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Glutamyl-tRNA reductase (hemA) CSB-YP774202DSL
CSB-EP774202DSL
CSB-BP774202DSL
CSB-MP774202DSL
CSB-EP774202DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae 30S ribosomal protein S4 (rpsD) CSB-YP774203DSL
CSB-EP774203DSL
CSB-BP774203DSL
CSB-MP774203DSL
CSB-EP774203DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Polymerase basic protein 2 (PB2), partial CSB-YP774204IEB
CSB-EP774204IEB
CSB-BP774204IEB
CSB-MP774204IEB
CSB-EP774204IEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian infectious bronchitis virus Nucleoprotein (N) CSB-YP774205ARW
CSB-EP774205ARW
CSB-BP774205ARW
CSB-EP774205ARW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Infectious hematopoietic necrosis virus Phosphoprotein (P) CSB-YP774206ICAJ
CSB-EP774206ICAJ
CSB-BP774206ICAJ
CSB-MP774206ICAJ
CSB-EP774206ICAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP774207SNN
CSB-EP774207SNN
CSB-BP774207SNN
CSB-MP774207SNN
CSB-EP774207SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable DNA ligase (lig), partial CSB-YP774208SNN
CSB-EP774208SNN
CSB-BP774208SNN
CSB-MP774208SNN
CSB-EP774208SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces avermitilis Urease subunit beta (ureB) CSB-YP774209SNN
CSB-EP774209SNN
CSB-BP774209SNN
CSB-MP774209SNN
CSB-EP774209SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uracil-DNA glycosylase 2 (ung2) CSB-YP774210SNN
CSB-EP774210SNN
CSB-BP774210SNN
CSB-MP774210SNN
CSB-EP774210SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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