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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chlamydophila caviae Inorganic pyrophosphatase (ppa) CSB-YP774171DSL
CSB-EP774171DSL
CSB-BP774171DSL
CSB-MP774171DSL
CSB-EP774171DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae 50S ribosomal protein L25 (rplY) CSB-YP774172DSL
CSB-EP774172DSL
CSB-BP774172DSL
CSB-MP774172DSL
CSB-EP774172DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Lipid-A-disaccharide synthase (lpxB), partial CSB-YP774173DSL
CSB-EP774173DSL
CSB-BP774173DSL
CSB-MP774173DSL
CSB-EP774173DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae 50S ribosomal protein L20 (rplT) CSB-YP774174DSL
CSB-EP774174DSL
CSB-BP774174DSL
CSB-MP774174DSL
CSB-EP774174DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Malate dehydrogenase (mdh) CSB-YP774175DSL
CSB-EP774175DSL
CSB-BP774175DSL
CSB-MP774175DSL
CSB-EP774175DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Dihydrodipicolinate synthase (dapA) CSB-YP774176DSL
CSB-EP774176DSL
CSB-BP774176DSL
CSB-MP774176DSL
CSB-EP774176DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae 50S ribosomal protein L10 (rplJ) CSB-YP774177DSL
CSB-EP774177DSL
CSB-BP774177DSL
CSB-MP774177DSL
CSB-EP774177DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae V-type ATP synthase subunit E (atpE) CSB-YP774178DSL
CSB-EP774178DSL
CSB-BP774178DSL
CSB-MP774178DSL
CSB-EP774178DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae 50S ribosomal protein L31 type B (rpmE2) CSB-YP774179DSL
CSB-EP774179DSL
CSB-BP774179DSL
CSB-MP774179DSL
CSB-EP774179DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Methionine--tRNA ligase (metG), partial CSB-YP774180DSL
CSB-EP774180DSL
CSB-BP774180DSL
CSB-MP774180DSL
CSB-EP774180DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP774181DSL
CSB-EP774181DSL
CSB-BP774181DSL
CSB-MP774181DSL
CSB-EP774181DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Tryptophan synthase beta chain 2 (trpB2) CSB-YP774182DSL
CSB-EP774182DSL
CSB-BP774182DSL
CSB-MP774182DSL
CSB-EP774182DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Adenylate kinase (adk) CSB-YP774183DSL
CSB-EP774183DSL
CSB-BP774183DSL
CSB-MP774183DSL
CSB-EP774183DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Acyl carrier protein (acpP) CSB-YP774184DSL
CSB-EP774184DSL
CSB-BP774184DSL
CSB-MP774184DSL
CSB-EP774184DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Bifunctional protein FolD (folD) CSB-YP774185DSL
CSB-EP774185DSL
CSB-BP774185DSL
CSB-MP774185DSL
CSB-EP774185DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Probable cytosol aminopeptidase (pepA) CSB-YP774186DSL
CSB-EP774186DSL
CSB-BP774186DSL
CSB-MP774186DSL
CSB-EP774186DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP774187DSL
CSB-EP774187DSL
CSB-BP774187DSL
CSB-MP774187DSL
CSB-EP774187DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Ribonuclease 3 (rnc) CSB-YP774188DSL
CSB-EP774188DSL
CSB-BP774188DSL
CSB-MP774188DSL
CSB-EP774188DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Maf-like protein CCA_00314 (CCA_00314) CSB-YP774189DSL
CSB-EP774189DSL
CSB-BP774189DSL
CSB-MP774189DSL
CSB-EP774189DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Ribonuclease HIII (rnhC) CSB-YP774190DSL
CSB-EP774190DSL
CSB-BP774190DSL
CSB-MP774190DSL
CSB-EP774190DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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