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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Dictyostelium discoideum BEACH domain-containing protein lvsB (lvsB), partial CSB-YP774772DKK
CSB-EP774772DKK
CSB-BP774772DKK
CSB-MP774772DKK
CSB-EP774772DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum DCN1-like protein 2 (DDB_G0272016) CSB-YP774773DKK
CSB-EP774773DKK
CSB-BP774773DKK
CSB-MP774773DKK
CSB-EP774773DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Copine-B (cpnB-1), partial CSB-YP774774DKK
CSB-EP774774DKK
CSB-BP774774DKK
CSB-MP774774DKK
CSB-EP774774DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Isopentenyl transferase (iptA) CSB-YP774775DKK
CSB-EP774775DKK
CSB-BP774775DKK
CSB-MP774775DKK
CSB-EP774775DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum BolA-like protein DDB_G0274169 (DDB_G0274169) CSB-YP774776DKK
CSB-EP774776DKK
CSB-BP774776DKK
CSB-MP774776DKK
CSB-EP774776DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Sphingosine kinase A (sgkA), partial CSB-YP774777DKK
CSB-EP774777DKK
CSB-BP774777DKK
CSB-MP774777DKK
CSB-EP774777DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative transmembrane protein DDB_G0277665 (DDB_G0277665), partial CSB-YP774778DKK1
CSB-EP774778DKK1
CSB-BP774778DKK1
CSB-MP774778DKK1
CSB-EP774778DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable valine--tRNA ligase, cytoplasmic (valS1), partial CSB-YP774779DKK
CSB-EP774779DKK
CSB-BP774779DKK
CSB-MP774779DKK
CSB-EP774779DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum TNF receptor-associated factor family protein DDB_G0272829 (DDB_G0272829), partial CSB-YP774780DKK
CSB-EP774780DKK
CSB-BP774780DKK
CSB-MP774780DKK
CSB-EP774780DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori Pro-corazonin (crz) CSB-YP774781BTT
CSB-EP774781BTT
CSB-BP774781BTT
CSB-MP774781BTT
CSB-EP774781BTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein king tubby (king-tubby) CSB-YP774782DLU
CSB-EP774782DLU
CSB-BP774782DLU
CSB-MP774782DLU
CSB-EP774782DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistosoma mansoni Acidic leucine-rich nuclear phosphoprotein 32-related protein (SMV0119) CSB-YP774783SXQ
CSB-EP774783SXQ
CSB-BP774783SXQ
CSB-MP774783SXQ
CSB-EP774783SXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Centruroides exilicauda Potassium channel toxin gamma-KTx 1.6 (ERG1) CSB-YP774784CFAY
CSB-EP774784CFAY
CSB-BP774784CFAY
CSB-MP774784CFAY
CSB-EP774784CFAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Centruroides sculpturatus Potassium channel toxin gamma-KTx 1.4 (ERG1) CSB-YP774785CGD
CSB-EP774785CGD
CSB-BP774785CGD
CSB-MP774785CGD
CSB-EP774785CGD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Centruroides gracilis Potassium channel toxin gamma-KTx 3.4 (ERG2) CSB-YP774786CFX
CSB-EP774786CFX
CSB-BP774786CFX
CSB-MP774786CFX
CSB-EP774786CFX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Centruroides noxius Potassium channel toxin gamma-KTx 4.11 (ERG4) CSB-YP774787DRB
CSB-EP774787DRB
CSB-BP774787DRB
CSB-MP774787DRB
CSB-EP774787DRB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Manduca sexta Putative defense protein Hdd11-like CSB-YP774788MPV
CSB-EP774788MPV
CSB-BP774788MPV
CSB-MP774788MPV
CSB-EP774788MPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Androctonus amoreuxi Toxin Aam2 CSB-YP774789AJY
CSB-EP774789AJY
CSB-BP774789AJY
CSB-MP774789AJY
CSB-EP774789AJY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Nucleoplasmin-2 (NPM2) CSB-YP774790HU
CSB-EP774790HU
CSB-BP774790HU
CSB-MP774790HU
CSB-EP774790HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative testis-specific prion protein (PRNT) CSB-YP774791HU
CSB-EP774791HU
CSB-BP774791HU
CSB-MP774791HU
CSB-EP774791HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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