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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana B3 domain-containing protein REM1 (REM1), partial CSB-YP801011DOA
CSB-EP801011DOA
CSB-BP801011DOA
CSB-MP801011DOA
CSB-EP801011DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein DEHYDRATION-INDUCED 19 homolog 3 (DI19-3) CSB-YP801012DOA
CSB-EP801012DOA
CSB-BP801012DOA
CSB-MP801012DOA
CSB-EP801012DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable mannose-1-phosphate guanylyltransferase 1 (Os03g0268400, LOC_Os03g16150) CSB-YP801013OFG
CSB-EP801013OFG
CSB-BP801013OFG
CSB-MP801013OFG
CSB-EP801013OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At4g21705, mitochondrial (At4g21705) CSB-YP801015DOA
CSB-EP801015DOA
CSB-BP801015DOA
CSB-MP801015DOA
CSB-EP801015DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase HOS1 (HOS1), partial CSB-YP801016DOA
CSB-EP801016DOA
CSB-BP801016DOA
CSB-MP801016DOA
CSB-EP801016DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase SINA-like 10 (At5g37930) CSB-YP801017DOA
CSB-EP801017DOA
CSB-BP801017DOA
CSB-MP801017DOA
CSB-EP801017DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crocus sativus Carotenoid 9,10 (9',10')-cleavage dioxygenase (CCD), partial CSB-YP801018CFAC
CSB-EP801018CFAC
CSB-BP801018CFAC
CSB-MP801018CFAC
CSB-EP801018CFAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative DNA repair protein RAD23-2 (RAD23-2) CSB-YP801020DOA
CSB-EP801020DOA
CSB-BP801020DOA
CSB-MP801020DOA
CSB-EP801020DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana attenuata 5-epi-aristolochene synthase 3, partial CSB-YP801021NBAZ
CSB-EP801021NBAZ
CSB-BP801021NBAZ
CSB-MP801021NBAZ
CSB-EP801021NBAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable cellulose synthase A catalytic subunit 2 [UDP-forming] (CESA2), partial CSB-YP801022OFG
CSB-EP801022OFG
CSB-BP801022OFG
CSB-MP801022OFG
CSB-EP801022OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Type I inositol 1,4,5-trisphosphate 5-phosphatase 1 (IP5P1), partial CSB-YP801023DOA
CSB-EP801023DOA
CSB-BP801023DOA
CSB-MP801023DOA
CSB-EP801023DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 1-aminocyclopropane-1-carboxylate oxidase homolog 1 (At1g06620) CSB-YP801024DOA
CSB-EP801024DOA
CSB-BP801024DOA
CSB-MP801024DOA
CSB-EP801024DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable potassium transporter 16 (HAK16), partial CSB-YP801025OFG
CSB-EP801025OFG
CSB-BP801025OFG
CSB-MP801025OFG
CSB-EP801025OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable histone H2A.3 (Os08g0427700, LOC_Os08g33100) CSB-YP801026OFG
CSB-EP801026OFG
CSB-BP801026OFG
CSB-MP801026OFG
CSB-EP801026OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 4-coumarate--CoA ligase-like 9 (4CLL9), partial CSB-YP801027DOA
CSB-EP801027DOA
CSB-BP801027DOA
CSB-MP801027DOA
CSB-EP801027DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Endoglucanase 21 (GLU9), partial CSB-YP801029OFG
CSB-EP801029OFG
CSB-BP801029OFG
CSB-MP801029OFG
CSB-EP801029OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable pectinesterase/pectinesterase inhibitor 36 (PME36) CSB-YP801030DOA
CSB-EP801030DOA
CSB-BP801030DOA
CSB-MP801030DOA
CSB-EP801030DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/LRR-repeat protein At2g29930 (At2g29930) CSB-YP801031DOA
CSB-EP801031DOA
CSB-BP801031DOA
CSB-MP801031DOA
CSB-EP801031DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable E3 ubiquitin-protein ligase ARI7 (ARI7), partial CSB-YP801032DOA
CSB-EP801032DOA
CSB-BP801032DOA
CSB-MP801032DOA
CSB-EP801032DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Protein Brevis radix-like 4 (BRXL4) CSB-YP801033OFG
CSB-EP801033OFG
CSB-BP801033OFG
CSB-MP801033OFG
CSB-EP801033OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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