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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rat Origin recognition complex subunit 1 (Orc1), partial CSB-YP800370RA
CSB-EP800370RA
CSB-BP800370RA
CSB-MP800370RA
CSB-EP800370RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Homeobox protein NANOG (Nanog) CSB-YP800371MO
CSB-EP800371MO
CSB-BP800371MO
CSB-MP800371MO
CSB-EP800371MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tamias striatus Guanine nucleotide-binding protein subunit beta-5 (GNB5) CSB-YP800372TLE
CSB-EP800372TLE
CSB-BP800372TLE
CSB-MP800372TLE
CSB-EP800372TLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Amphoterin-induced protein 1 (Amigo1), partial CSB-YP800373RA
CSB-EP800373RA
CSB-BP800373RA
CSB-MP800373RA
CSB-EP800373RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Inositol-trisphosphate 3-kinase C (Itpkc) CSB-YP800375RA
CSB-EP800375RA
CSB-BP800375RA
CSB-MP800375RA
CSB-EP800375RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ras-related protein Rap-2a (Rap2a) CSB-YP800376MO
CSB-EP800376MO
CSB-BP800376MO
CSB-MP800376MO
CSB-EP800376MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Transcription initiation factor IIA subunit 2 (Gtf2a2) CSB-YP800377MO
CSB-EP800377MO
CSB-BP800377MO
CSB-MP800377MO
CSB-EP800377MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein FAM206A (Fam206a) CSB-YP800378MO
CSB-EP800378MO
CSB-BP800378MO
CSB-MP800378MO
CSB-EP800378MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ribonuclease H2 subunit B (Rnaseh2b) CSB-YP800379MO
CSB-EP800379MO
CSB-BP800379MO
CSB-MP800379MO
CSB-EP800379MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein E7 (E7) CSB-YP800380HOA
CSB-EP800380HOA
CSB-BP800380HOA
CSB-MP800380HOA
CSB-EP800380HOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse ATP-dependent RNA helicase DDX42 (Ddx42), partial CSB-YP800381MO
CSB-EP800381MO
CSB-BP800381MO
CSB-MP800381MO
CSB-EP800381MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Glutamate-rich WD repeat-containing protein 1 (Grwd1) CSB-YP800382MO
CSB-EP800382MO
CSB-BP800382MO
CSB-MP800382MO
CSB-EP800382MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Glucoside xylosyltransferase 2 (Gxylt2), partial CSB-YP800383MO
CSB-EP800383MO
CSB-BP800383MO
CSB-MP800383MO
CSB-EP800383MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Uncharacterized protein C12orf60 homolog CSB-YP800384MO
CSB-EP800384MO
CSB-BP800384MO
CSB-MP800384MO
CSB-EP800384MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse G2/mitotic-specific cyclin-B3 (Ccnb3), partial CSB-YP800385MO
CSB-EP800385MO
CSB-BP800385MO
CSB-MP800385MO
CSB-EP800385MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse C-X-C chemokine receptor type 1 (Cxcr1), partial CSB-YP800386MO1
CSB-EP800386MO1
CSB-BP800386MO1
CSB-MP800386MO1
CSB-EP800386MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hepatitis B virus genotype C subtype adr Large envelope protein (S), partial CSB-YP800387HEAA1
CSB-EP800387HEAA1
CSB-BP800387HEAA1
CSB-MP800387HEAA1
CSB-EP800387HEAA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Proline-rich protein 3 (Prr3) CSB-YP800388MO
CSB-EP800388MO
CSB-BP800388MO
CSB-MP800388MO
CSB-EP800388MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein PTHB1 (Bbs9), partial CSB-YP800389MO
CSB-EP800389MO
CSB-BP800389MO
CSB-MP800389MO
CSB-EP800389MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Peroxisome proliferator-activated receptor gamma coactivator 1-beta (Ppargc1b), partial CSB-YP800390RA
CSB-EP800390RA
CSB-BP800390RA
CSB-MP800390RA
CSB-EP800390RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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