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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas putida Protein grpE (grpE) CSB-YP801575FGB
CSB-EP801575FGB
CSB-BP801575FGB
CSB-MP801575FGB
CSB-EP801575FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Phosphoglucosamine mutase (glmM) CSB-YP801576FGB
CSB-EP801576FGB
CSB-BP801576FGB
CSB-MP801576FGB
CSB-EP801576FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP801577FGB
CSB-EP801577FGB
CSB-BP801577FGB
CSB-MP801577FGB
CSB-EP801577FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Sulfoxide reductase heme-binding subunit YedZ (yedZ), partial CSB-YP801578FGB1
CSB-EP801578FGB1
CSB-BP801578FGB1
CSB-MP801578FGB1
CSB-EP801578FGB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP801579FGB
CSB-EP801579FGB
CSB-BP801579FGB
CSB-MP801579FGB
CSB-EP801579FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Peptide deformylase 2 (def2) CSB-YP801580FGB
CSB-EP801580FGB
CSB-BP801580FGB
CSB-MP801580FGB
CSB-EP801580FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Alanine--tRNA ligase (alaS), partial CSB-YP801581FGB
CSB-EP801581FGB
CSB-BP801581FGB
CSB-MP801581FGB
CSB-EP801581FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Cytochrome c biogenesis ATP-binding export protein CcmA (ccmA) CSB-YP801582FGB
CSB-EP801582FGB
CSB-BP801582FGB
CSB-MP801582FGB
CSB-EP801582FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UPF0133 protein PP_4268 (PP_4268) CSB-YP801583FGB
CSB-EP801583FGB
CSB-BP801583FGB
CSB-MP801583FGB
CSB-EP801583FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP801584FGB
CSB-EP801584FGB
CSB-BP801584FGB
CSB-MP801584FGB
CSB-EP801584FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UvrABC system protein C (uvrC), partial CSB-YP801585FGB
CSB-EP801585FGB
CSB-BP801585FGB
CSB-MP801585FGB
CSB-EP801585FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida NADH pyrophosphatase (nudC) CSB-YP801586FGB
CSB-EP801586FGB
CSB-BP801586FGB
CSB-MP801586FGB
CSB-EP801586FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Siroheme synthase (cysG) CSB-YP801587FGB
CSB-EP801587FGB
CSB-BP801587FGB
CSB-MP801587FGB
CSB-EP801587FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Molybdenum cofactor guanylyltransferase (mobA) CSB-YP801588FGB
CSB-EP801588FGB
CSB-BP801588FGB
CSB-MP801588FGB
CSB-EP801588FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Error-prone DNA polymerase (dnaE2), partial CSB-YP801589FGB
CSB-EP801589FGB
CSB-BP801589FGB
CSB-MP801589FGB
CSB-EP801589FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UPF0145 protein PP_2873 (PP_2873) CSB-YP801590FGB
CSB-EP801590FGB
CSB-BP801590FGB
CSB-MP801590FGB
CSB-EP801590FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Urease subunit alpha (ureC), partial CSB-YP801591FGB
CSB-EP801591FGB
CSB-BP801591FGB
CSB-MP801591FGB
CSB-EP801591FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Coenzyme PQQ synthesis protein D 2 (pqqD2) CSB-YP801592FGB
CSB-EP801592FGB
CSB-BP801592FGB
CSB-MP801592FGB
CSB-EP801592FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Translation initiation factor IF-3 (infC) CSB-YP801593FGB
CSB-EP801593FGB
CSB-BP801593FGB
CSB-MP801593FGB
CSB-EP801593FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Probable sugar efflux transporter (sotB), partial CSB-YP801594FGB
CSB-EP801594FGB
CSB-BP801594FGB
CSB-MP801594FGB
CSB-EP801594FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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