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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Glycine--tRNA ligase (glyQS) CSB-YP800571BQE
CSB-EP800571BQE
CSB-BP800571BQE
CSB-MP800571BQE
CSB-EP800571BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0061 protein BA_3567/GBAA_3567/BAS3307 (BA_3567, GBAA_3567, BAS3307) CSB-YP800572BQE
CSB-EP800572BQE
CSB-BP800572BQE
CSB-MP800572BQE
CSB-EP800572BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate-1-semialdehyde 2,1-aminomutase 1 (hemL1) CSB-YP800573BQE
CSB-EP800573BQE
CSB-BP800573BQE
CSB-MP800573BQE
CSB-EP800573BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thiamine-phosphate synthase (thiE) CSB-YP800574BQE
CSB-EP800574BQE
CSB-BP800574BQE
CSB-MP800574BQE
CSB-EP800574BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized RNA methyltransferase BA_0333/GBAA_0333/BAS0318 (BA_0333, GBAA_0333, BAS0318) CSB-YP800575BQE
CSB-EP800575BQE
CSB-BP800575BQE
CSB-MP800575BQE
CSB-EP800575BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heptaprenylglyceryl phosphate synthase (pcrB) CSB-YP800576BQE
CSB-EP800576BQE
CSB-BP800576BQE
CSB-MP800576BQE
CSB-EP800576BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Elongation factor Tu (tuf1) CSB-YP800577NHH
CSB-EP800577NHH
CSB-BP800577NHH
CSB-MP800577NHH
CSB-EP800577NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP800578SNN
CSB-EP800578SNN
CSB-BP800578SNN
CSB-MP800578SNN
CSB-EP800578SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Ribosomal protein L11 methyltransferase (prmA) CSB-YP800579NHH
CSB-EP800579NHH
CSB-BP800579NHH
CSB-MP800579NHH
CSB-EP800579NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural glycoprotein 4, partial CSB-YP800580ROH
CSB-EP800580ROH
CSB-BP800580ROH
CSB-MP800580ROH
CSB-EP800580ROH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 1 CSB-YP800581ROH
CSB-EP800581ROH
CSB-BP800581ROH
CSB-MP800581ROH
CSB-EP800581ROH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus C Non-structural glycoprotein 4, partial CSB-YP800582RHJ
CSB-EP800582RHJ
CSB-BP800582RHJ
CSB-MP800582RHJ
CSB-EP800582RHJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Ribosomal protein L11 methyltransferase (prmA) CSB-YP800583ELW
CSB-EP800583ELW
CSB-BP800583ELW
CSB-MP800583ELW
CSB-EP800583ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP800584NHH
CSB-EP800584NHH
CSB-BP800584NHH
CSB-MP800584NHH
CSB-EP800584NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP800585DSL
CSB-EP800585DSL
CSB-BP800585DSL
CSB-MP800585DSL
CSB-EP800585DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 10 kDa chaperonin (groS) CSB-YP800586SNN
CSB-EP800586SNN
CSB-BP800586SNN
CSB-MP800586SNN
CSB-EP800586SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Methionyl-tRNA formyltransferase (fmt) CSB-YP800587NHH
CSB-EP800587NHH
CSB-BP800587NHH
CSB-MP800587NHH
CSB-EP800587NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Glutamate 5-kinase (proB) CSB-YP800588NHH
CSB-EP800588NHH
CSB-BP800588NHH
CSB-MP800588NHH
CSB-EP800588NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea tRNA pseudouridine synthase B (truB) CSB-YP800589NHH
CSB-EP800589NHH
CSB-BP800589NHH
CSB-MP800589NHH
CSB-EP800589NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Translation initiation factor IF-1 (infA) CSB-YP800590NHH
CSB-EP800590NHH
CSB-BP800590NHH
CSB-MP800590NHH
CSB-EP800590NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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