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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Danio rerio U3 small nucleolar RNA-associated protein 15 homolog (utp15), partial CSB-YP801872DIL
CSB-EP801872DIL
CSB-BP801872DIL
CSB-MP801872DIL
CSB-EP801872DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Methylsterol monooxygenase 1 (msmo1), partial CSB-YP801873DIL1
CSB-EP801873DIL1
CSB-BP801873DIL1
CSB-MP801873DIL1
CSB-EP801873DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio La-related protein 7 (larp7), partial CSB-YP801874DIL
CSB-EP801874DIL
CSB-BP801874DIL
CSB-MP801874DIL
CSB-EP801874DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Oligosaccharyltransferase complex subunit ostc (ostc), partial CSB-YP801875DIL1
CSB-EP801875DIL1
CSB-BP801875DIL1
CSB-MP801875DIL1
CSB-EP801875DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis E3 ubiquitin-protein ligase RING2-B (rnf2-b) CSB-YP801876XBE
CSB-EP801876XBE
CSB-BP801876XBE
CSB-MP801876XBE
CSB-EP801876XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Cysteine--tRNA ligase, cytoplasmic (cars), partial CSB-YP801877XBE
CSB-EP801877XBE
CSB-BP801877XBE
CSB-MP801877XBE
CSB-EP801877XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Midnolin-B (midn-b) CSB-YP801878XBE
CSB-EP801878XBE
CSB-BP801878XBE
CSB-MP801878XBE
CSB-EP801878XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis OTU domain-containing protein 5-A (otud5-a), partial CSB-YP801879XBE
CSB-EP801879XBE
CSB-BP801879XBE
CSB-MP801879XBE
CSB-EP801879XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Guanine nucleotide-binding protein-like 3 (gnl3), partial CSB-YP801880XBE
CSB-EP801880XBE
CSB-BP801880XBE
CSB-MP801880XBE
CSB-EP801880XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Heterogeneous nuclear ribonucleoprotein D-like-A (hnrpdl-a) CSB-YP801881XBE
CSB-EP801881XBE
CSB-BP801881XBE
CSB-MP801881XBE
CSB-EP801881XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Carnitine O-palmitoyltransferase 2, mitochondrial (cpt2), partial CSB-YP801882XBE
CSB-EP801882XBE
CSB-BP801882XBE
CSB-MP801882XBE
CSB-EP801882XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Twinfilin-2-A (twf2-a) CSB-YP801883XBE
CSB-EP801883XBE
CSB-BP801883XBE
CSB-MP801883XBE
CSB-EP801883XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Ubiquitin carboxyl-terminal hydrolase 10-B (usp10-b), partial CSB-YP801884XBE
CSB-EP801884XBE
CSB-BP801884XBE
CSB-MP801884XBE
CSB-EP801884XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Zinc finger protein 36, C3H1 type-like 2-A (zfp36l2-A) CSB-YP801885XBE
CSB-EP801885XBE
CSB-BP801885XBE
CSB-MP801885XBE
CSB-EP801885XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis U3 small nucleolar RNA-associated protein 15 homolog (utp15), partial CSB-YP801886XBE
CSB-EP801886XBE
CSB-BP801886XBE
CSB-MP801886XBE
CSB-EP801886XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Sepiapterin reductase (spr) CSB-YP801887XBE
CSB-EP801887XBE
CSB-BP801887XBE
CSB-MP801887XBE
CSB-EP801887XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein NDRG2 (ndrg2) CSB-YP801888XBE
CSB-EP801888XBE
CSB-BP801888XBE
CSB-MP801888XBE
CSB-EP801888XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis LMBR1 domain-containing protein 2 (lmbrd2), partial CSB-YP801889XBE
CSB-EP801889XBE
CSB-BP801889XBE
CSB-MP801889XBE
CSB-EP801889XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Ankyrin repeat domain-containing protein 13C-B (ankrd13c-b), partial CSB-YP801890XBE
CSB-EP801890XBE
CSB-BP801890XBE
CSB-MP801890XBE
CSB-EP801890XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Acylglycerol kinase, mitochondrial (agk) CSB-YP801891XBE
CSB-EP801891XBE
CSB-BP801891XBE
CSB-MP801891XBE
CSB-EP801891XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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