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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Eimeria tenella 50S ribosomal protein L14, apicoplast (rpl14) CSB-YP801768EJU
CSB-EP801768EJU
CSB-BP801768EJU
CSB-MP801768EJU
CSB-EP801768EJU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borszczowia aralocaspica Photosystem II reaction center protein T (psbT), partial CSB-YP801769BEAD
CSB-EP801769BEAD
CSB-BP801769BEAD
CSB-MP801769BEAD
CSB-EP801769BEAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Panax ginseng Photosystem II reaction center protein M (psbM), partial CSB-YP801770EQW
CSB-EP801770EQW
CSB-BP801770EQW
CSB-MP801770EQW
CSB-EP801770EQW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Mediator of RNA polymerase II transcription subunit 12 (MED12), partial CSB-YP801771EQV
CSB-EP801771EQV
CSB-BP801771EQV
CSB-MP801771EQV
CSB-EP801771EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus Rab GDP dissociation inhibitor alpha (GDI1) CSB-YP801772EXP
CSB-EP801772EXP
CSB-BP801772EXP
CSB-MP801772EXP
CSB-EP801772EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorocebus aethiops DNA dC->dU-editing enzyme APOBEC-3G (APOBEC3G) CSB-YP801773DSU
CSB-EP801773DSU
CSB-BP801773DSU
CSB-MP801773DSU
CSB-EP801773DSU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Tripartite motif-containing protein 26 (TRIM26), partial CSB-YP801774EQV
CSB-EP801774EQV
CSB-BP801774EQV
CSB-MP801774EQV
CSB-EP801774EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Psoriasis susceptibility 1 candidate gene 1 protein homolog (PSORS1C1) CSB-YP801775EQV
CSB-EP801775EQV
CSB-BP801775EQV
CSB-MP801775EQV
CSB-EP801775EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine 39S ribosomal protein L12, mitochondrial (MRPL12) CSB-YP801776BO
CSB-EP801776BO
CSB-BP801776BO
CSB-MP801776BO
CSB-EP801776BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca mulatta FUN14 domain-containing protein 2 (FUNDC2) CSB-YP801777MOW
CSB-EP801777MOW
CSB-BP801777MOW
CSB-MP801777MOW
CSB-EP801777MOW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog AP-3 complex subunit beta-1 (AP3B1), partial CSB-YP801778DO
CSB-EP801778DO
CSB-BP801778DO
CSB-MP801778DO
CSB-EP801778DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus Ribonuclease-like protein 9 (RNASE9) CSB-YP801779EXP
CSB-EP801779EXP
CSB-BP801779EXP
CSB-MP801779EXP
CSB-EP801779EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus Cytochrome c oxidase subunit 6C (COX6C), partial CSB-YP801780EXP1
CSB-EP801780EXP1
CSB-BP801780EXP1
CSB-MP801780EXP1
CSB-EP801780EXP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig T-cell surface glycoprotein CD3 epsilon chain (CD3E), partial CSB-YP801781PI
CSB-BP801781PI
CSB-MP801781PI
CSB-EP801781PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cat Malate dehydrogenase, cytoplasmic (MDH1) CSB-YP801782CA
CSB-EP801782CA
CSB-BP801782CA
CSB-MP801782CA
CSB-EP801782CA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Granzyme A (GZMA) CSB-YP801783BO
CSB-EP801783BO
CSB-BP801783BO
CSB-MP801783BO
CSB-EP801783BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Trans-2-enoyl-CoA reductase, mitochondrial (MECR) CSB-YP801784BO
CSB-EP801784BO
CSB-BP801784BO
CSB-MP801784BO
CSB-EP801784BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Protein TBRG4 (TBRG4), partial CSB-YP801785PI
CSB-EP801785PI
CSB-BP801785PI
CSB-MP801785PI
CSB-EP801785PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Centruroides limpidus limpidus Toxin Cll5c CSB-YP801786CGA
CSB-EP801786CGA
CSB-BP801786CGA
CSB-MP801786CGA
CSB-EP801786CGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Transmembrane GTPase Marf (Marf), partial CSB-YP801787DLU
CSB-EP801787DLU
CSB-BP801787DLU
CSB-MP801787DLU
CSB-EP801787DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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