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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse Dedicator of cytokinesis protein 10 (Dock10), partial CSB-YP803971MO
CSB-EP803971MO
CSB-BP803971MO
CSB-MP803971MO
CSB-EP803971MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 (Dhx32) CSB-YP803972MO
CSB-EP803972MO
CSB-BP803972MO
CSB-MP803972MO
CSB-EP803972MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Probable G-protein coupled receptor 61 (Gpr61), partial CSB-YP803973MO1
CSB-EP803973MO1
CSB-BP803973MO1
CSB-MP803973MO1
CSB-EP803973MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Probable tRNA pseudouridine synthase 1 (Trub1) CSB-YP803974MO
CSB-EP803974MO
CSB-BP803974MO
CSB-MP803974MO
CSB-EP803974MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Tripartite motif-containing protein 47 (Trim47) CSB-YP803975MO
CSB-EP803975MO
CSB-BP803975MO
CSB-MP803975MO
CSB-EP803975MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ankyrin repeat domain-containing protein SOWAHC (Sowahc) CSB-YP803976MO
CSB-EP803976MO
CSB-BP803976MO
CSB-MP803976MO
CSB-EP803976MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse L-asparaginase (Asrgl1) CSB-YP803977MO
CSB-EP803977MO
CSB-BP803977MO
CSB-MP803977MO
CSB-EP803977MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Tetratricopeptide repeat protein 34 (Ttc34) CSB-YP803978MO
CSB-EP803978MO
CSB-BP803978MO
CSB-MP803978MO
CSB-EP803978MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse DIS3-like exonuclease 1 (Dis3l), partial CSB-YP803979MO
CSB-EP803979MO
CSB-BP803979MO
CSB-MP803979MO
CSB-EP803979MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Serine/threonine-protein kinase tousled-like 1 (Tlk1) CSB-YP803980MO
CSB-EP803980MO
CSB-BP803980MO
CSB-MP803980MO
CSB-EP803980MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Homeobox protein TGIF2 (Tgif2) CSB-YP803981MO
CSB-EP803981MO
CSB-BP803981MO
CSB-MP803981MO
CSB-EP803981MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse SH3 domain-containing RING finger protein 3 (Sh3rf3), partial CSB-YP803982MO
CSB-EP803982MO
CSB-BP803982MO
CSB-MP803982MO
CSB-EP803982MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein FAM46B (Fam46b) CSB-YP803983MO
CSB-EP803983MO
CSB-BP803983MO
CSB-MP803983MO
CSB-EP803983MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Copine-1 (Cpne1) CSB-YP803984MO
CSB-EP803984MO
CSB-BP803984MO
CSB-MP803984MO
CSB-EP803984MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Methionine synthase reductase (Mtrr) CSB-YP803985MO
CSB-EP803985MO
CSB-BP803985MO
CSB-MP803985MO
CSB-EP803985MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse BPI fold-containing family B member 2 (Bpifb2) CSB-YP803986MO
CSB-EP803986MO
CSB-BP803986MO
CSB-MP803986MO
CSB-EP803986MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse UPF0640 protein C3orf78 homolog, partial CSB-YP803987MO1
CSB-EP803987MO1
CSB-BP803987MO1
CSB-MP803987MO1
CSB-EP803987MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse RNA (guanine-9-)-methyltransferase domain-containing protein 2 (Rg9mtd2) CSB-YP803988MO
CSB-EP803988MO
CSB-BP803988MO
CSB-MP803988MO
CSB-EP803988MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Histone-lysine N-methyltransferase SETDB2 (Setdb2) CSB-YP803989MO
CSB-EP803989MO
CSB-BP803989MO
CSB-MP803989MO
CSB-EP803989MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse RAS protein activator like-3 (Rasal3), partial CSB-YP803990MO
CSB-EP803990MO
CSB-BP803990MO
CSB-MP803990MO
CSB-EP803990MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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