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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse Putative sodium-coupled neutral amino acid transporter 7 (Slc38a7), partial CSB-YP805975MO
CSB-EP805975MO
CSB-BP805975MO
CSB-MP805975MO
CSB-EP805975MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase (B3gnt1), partial CSB-YP805976MO
CSB-EP805976MO
CSB-BP805976MO
CSB-MP805976MO
CSB-EP805976MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ubiquitin carboxyl-terminal hydrolase 40 (Usp40), partial CSB-YP805977MO
CSB-EP805977MO
CSB-BP805977MO
CSB-MP805977MO
CSB-EP805977MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Probable tRNA threonylcarbamoyladenosine biosynthesis protein Osgep (Osgep) CSB-YP805978MO
CSB-EP805978MO
CSB-BP805978MO
CSB-MP805978MO
CSB-EP805978MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Metallo-beta-lactamase domain-containing protein 1 (Mblac1) CSB-YP805979MO
CSB-EP805979MO
CSB-BP805979MO
CSB-MP805979MO
CSB-EP805979MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Serine/threonine-protein phosphatase PGAM5, mitochondrial (Pgam5), partial CSB-YP805980MO
CSB-EP805980MO
CSB-BP805980MO
CSB-MP805980MO
CSB-EP805980MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse SKI family transcriptional corepressor 1 (Skor1), partial CSB-YP805981MO
CSB-EP805981MO
CSB-BP805981MO
CSB-MP805981MO
CSB-EP805981MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Cytosolic endo-beta-N-acetylglucosaminidase (Engase) CSB-YP805982MO
CSB-EP805982MO
CSB-BP805982MO
CSB-MP805982MO
CSB-EP805982MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Claudin-17 (Cldn17), partial CSB-YP805983MO1
CSB-EP805983MO1
CSB-BP805983MO1
CSB-MP805983MO1
CSB-EP805983MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein phosphatase 1K, mitochondrial (Ppm1k) CSB-YP805984MO
CSB-EP805984MO
CSB-BP805984MO
CSB-MP805984MO
CSB-EP805984MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Sodium leak channel non-selective protein (Nalcn), partial CSB-YP805985MO
CSB-EP805985MO
CSB-BP805985MO
CSB-MP805985MO
CSB-EP805985MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse BRI3-binding protein (Bri3bp), partial CSB-YP805986MO1
CSB-EP805986MO1
CSB-BP805986MO1
CSB-MP805986MO1
CSB-EP805986MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ubiquitin carboxyl-terminal hydrolase 47 (Usp47), partial CSB-YP805987MO
CSB-EP805987MO
CSB-BP805987MO
CSB-MP805987MO
CSB-EP805987MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Serine/threonine-protein kinase TAO3 (Taok3), partial CSB-YP805988MO
CSB-EP805988MO
CSB-BP805988MO
CSB-MP805988MO
CSB-EP805988MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse TBC1 domain family member 4 (Tbc1d4), partial CSB-YP805989MO
CSB-EP805989MO
CSB-BP805989MO
CSB-MP805989MO
CSB-EP805989MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Calcium-dependent secretion activator 2 (Cadps2), partial CSB-YP805990MO
CSB-EP805990MO
CSB-BP805990MO
CSB-MP805990MO
CSB-EP805990MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 (Arap2), partial CSB-YP805991MO
CSB-EP805991MO
CSB-BP805991MO
CSB-MP805991MO
CSB-EP805991MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Tensin-4 (Tns4) CSB-YP805992MO
CSB-EP805992MO
CSB-BP805992MO
CSB-MP805992MO
CSB-EP805992MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Leucine-rich repeat transmembrane neuronal protein 3 (Lrrtm3), partial CSB-YP805993MO
CSB-EP805993MO
CSB-BP805993MO
CSB-MP805993MO
CSB-EP805993MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Phosphoglucomutase-like protein 5 (Pgm5) CSB-YP805994MO
CSB-EP805994MO
CSB-BP805994MO
CSB-MP805994MO
CSB-EP805994MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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