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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant DNA translocase FtsK (ftsK), partial CSB-YP807723VFI
CSB-EP807723VFI
CSB-BP807723VFI
CSB-MP807723VFI
CSB-EP807723VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidinol dehydrogenase (hisD) CSB-YP807724VFI
CSB-EP807724VFI
CSB-BP807724VFI
CSB-MP807724VFI
CSB-EP807724VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP807725VFI
CSB-EP807725VFI
CSB-BP807725VFI
CSB-MP807725VFI
CSB-EP807725VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trimethylamine-N-oxide reductase (torA), partial CSB-YP807726VFI
CSB-EP807726VFI
CSB-BP807726VFI
CSB-MP807726VFI
CSB-EP807726VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative reductase VV1_2829 (VV1_2829) CSB-YP807727VFI
CSB-EP807727VFI
CSB-BP807727VFI
CSB-MP807727VFI
CSB-EP807727VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA large subunit methyltransferase I (rlmI) CSB-YP807728VFI
CSB-EP807728VFI
CSB-BP807728VFI
CSB-MP807728VFI
CSB-EP807728VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell division protein ZapC (zapC) CSB-YP807729VFI
CSB-EP807729VFI
CSB-BP807729VFI
CSB-MP807729VFI
CSB-EP807729VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Molybdenum cofactor guanylyltransferase (mobA) CSB-YP807730VFI
CSB-EP807730VFI
CSB-BP807730VFI
CSB-MP807730VFI
CSB-EP807730VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus atty acid metabolism regulator protein (fadR) CSB-YP807731VFI
CSB-EP807731VFI
CSB-BP807731VFI
CSB-MP807731VFI
CSB-EP807731VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP807732VFI
CSB-EP807732VFI
CSB-BP807732VFI
CSB-MP807732VFI
CSB-EP807732VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromosome partition protein mukE (mukE) CSB-YP807733VFI
CSB-EP807733VFI
CSB-BP807733VFI
CSB-MP807733VFI
CSB-EP807733VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal large subunit pseudouridine synthase E (rluE) CSB-YP807734VFI
CSB-EP807734VFI
CSB-BP807734VFI
CSB-MP807734VFI
CSB-EP807734VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipoprotein-releasing system ATP-binding protein LolD (lolD) CSB-YP807735VFI
CSB-EP807735VFI
CSB-BP807735VFI
CSB-MP807735VFI
CSB-EP807735VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP807736VFI
CSB-EP807736VFI
CSB-BP807736VFI
CSB-MP807736VFI
CSB-EP807736VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoheptose isomerase (gmhA) CSB-YP807737VFI
CSB-EP807737VFI
CSB-BP807737VFI
CSB-MP807737VFI
CSB-EP807737VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipid-A-disaccharide synthase (lpxB) CSB-YP807738VFI
CSB-EP807738VFI
CSB-BP807738VFI
CSB-MP807738VFI
CSB-EP807738VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0325 protein VV1_1856 (VV1_1856) CSB-YP807739VFI
CSB-EP807739VFI
CSB-BP807739VFI
CSB-MP807739VFI
CSB-EP807739VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Na (+)-translocating NADH-quinone reductase subunit B (nqrB), partial CSB-YP807740VFI
CSB-EP807740VFI
CSB-BP807740VFI
CSB-MP807740VFI
CSB-EP807740VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Purine nucleoside phosphorylase DeoD-type 1 (deoD1) CSB-YP807741VFI
CSB-EP807741VFI
CSB-BP807741VFI
CSB-MP807741VFI
CSB-EP807741VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome-binding factor A (rbfA) CSB-YP807742VFI
CSB-EP807742VFI
CSB-BP807742VFI
CSB-MP807742VFI
CSB-EP807742VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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