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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xenopus laevis Ras-like protein family member 11B (rasl11b) CSB-YP806783XBE
CSB-EP806783XBE
CSB-BP806783XBE
CSB-MP806783XBE
CSB-EP806783XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis G kinase-anchoring protein 1-B (gkap1-b) CSB-YP806784XBE
CSB-EP806784XBE
CSB-BP806784XBE
CSB-MP806784XBE
CSB-EP806784XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Alpha- (1,3)-fucosyltransferase 10 (FUT10), partial CSB-YP806785CH
CSB-EP806785CH
CSB-BP806785CH
CSB-MP806785CH
CSB-EP806785CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carassius cuvieri Metallothionein A (mta) CSB-YP806786CJAP
CSB-EP806786CJAP
CSB-BP806786CJAP
CSB-MP806786CJAP
CSB-EP806786CJAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Homeobox protein Hox-B7a (hoxb7a) CSB-YP806787DIL
CSB-EP806787DIL
CSB-BP806787DIL
CSB-MP806787DIL
CSB-EP806787DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Coiled-coil domain-containing protein 80 (CCDC80), partial CSB-YP806788CH
CSB-EP806788CH
CSB-BP806788CH
CSB-MP806788CH
CSB-EP806788CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Solute carrier family 35 member B1 (slc35b1), partial CSB-YP806789XBF
CSB-EP806789XBF
CSB-BP806789XBF
CSB-MP806789XBF
CSB-EP806789XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Hairy/enhancer-of-split related with YRPW motif protein 1 (hey1) CSB-YP806790DIL
CSB-EP806790DIL
CSB-BP806790DIL
CSB-MP806790DIL
CSB-EP806790DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hemitripterus americanus Protein transport protein Sec61 subunit alpha (sec61a), partial CSB-YP806791HDJ
CSB-EP806791HDJ
CSB-BP806791HDJ
CSB-MP806791HDJ
CSB-EP806791HDJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bungarus candidus Protease inhibitor B CSB-YP806792BPN
CSB-EP806792BPN
CSB-BP806792BPN
CSB-MP806792BPN
CSB-EP806792BPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bungarus candidus Beta-bungarotoxin B1 chain CSB-YP806793BPN
CSB-EP806793BPN
CSB-BP806793BPN
CSB-MP806793BPN
CSB-EP806793BPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis SHC-transforming protein 1 (shc1) CSB-YP806794XBE
CSB-EP806794XBE
CSB-BP806794XBE
CSB-MP806794XBE
CSB-EP806794XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viridovipera stejnegeri Snake venom serine protease 1 homolog CSB-YP806795VDN
CSB-EP806795VDN
CSB-BP806795VDN
CSB-MP806795VDN
CSB-EP806795VDN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Serine/threonine-protein kinase Chk1 (CHEK1) CSB-YP806796CH
CSB-EP806796CH
CSB-BP806796CH
CSB-MP806796CH
CSB-EP806796CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Red-sensitive opsin-2 (opn1lw2), partial CSB-YP806797DIL1
CSB-EP806797DIL1
CSB-BP806797DIL1
CSB-MP806797DIL1
CSB-EP806797DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tamiami virus Pre-glycoprotein polyprotein GP complex (GPC), partial CSB-YP806798TJY1
CSB-EP806798TJY1
CSB-BP806798TJY1
CSB-MP806798TJY1
CSB-EP806798TJY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicular stomatitis Indiana virus Phosphoprotein (P) CSB-YP806799VBE
CSB-EP806799VBE
CSB-BP806799VBE
CSB-MP806799VBE
CSB-EP806799VBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicular stomatitis Indiana virus Matrix protein (M) CSB-YP806800VBF
CSB-BP806800VBF
CSB-MP806800VBF
CSB-EP806800VBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabies virus Large structural protein (L), partial CSB-YP806801RAF
CSB-EP806801RAF
CSB-BP806801RAF
CSB-MP806801RAF
CSB-EP806801RAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rachiplusia ou multiple nucleopolyhedrovirus E3 ubiquitin-protein ligase IE2 (IE2) CSB-YP806802RCAX
CSB-EP806802RCAX
CSB-BP806802RCAX
CSB-MP806802RCAX
CSB-EP806802RCAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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