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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bradyrhizobium japonicum Dihydroxy-acid dehydratase 1 (ilvD1), partial CSB-YP806703BVW
CSB-EP806703BVW
CSB-BP806703BVW
CSB-MP806703BVW
CSB-EP806703BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum NAD-dependent protein deacetylase 1 (cobB1) CSB-YP806704BVW
CSB-EP806704BVW
CSB-BP806704BVW
CSB-MP806704BVW
CSB-EP806704BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Vitamin B12-dependent ribonucleotide reductase (nrdJ), partial CSB-YP806705BVW
CSB-EP806705BVW
CSB-BP806705BVW
CSB-MP806705BVW
CSB-EP806705BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP806706BVW
CSB-EP806706BVW
CSB-BP806706BVW
CSB-MP806706BVW
CSB-EP806706BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum GTPase Der (der) CSB-YP806707BVW
CSB-EP806707BVW
CSB-BP806707BVW
CSB-MP806707BVW
CSB-EP806707BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Ribonuclease Z (rnz) CSB-YP806708BVW
CSB-EP806708BVW
CSB-BP806708BVW
CSB-MP806708BVW
CSB-EP806708BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Lipoyl synthase 2 (lipA2) CSB-YP806709BVW
CSB-EP806709BVW
CSB-BP806709BVW
CSB-MP806709BVW
CSB-EP806709BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum D-alanine--D-alanine ligase B (ddlA) CSB-YP806710BVW
CSB-EP806710BVW
CSB-BP806710BVW
CSB-MP806710BVW
CSB-EP806710BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum UPF0324 membrane protein blr3189 (blr3189), partial CSB-YP806711BVW
CSB-EP806711BVW
CSB-BP806711BVW
CSB-MP806711BVW
CSB-EP806711BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP806712BVW
CSB-EP806712BVW
CSB-BP806712BVW
CSB-MP806712BVW
CSB-EP806712BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Protein CrcB homolog 2 (crcB2), partial CSB-YP806713BVW1
CSB-EP806713BVW1
CSB-BP806713BVW1
CSB-MP806713BVW1
CSB-EP806713BVW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Ribonuclease HII (rnhB) CSB-YP806714BVW
CSB-EP806714BVW
CSB-BP806714BVW
CSB-MP806714BVW
CSB-EP806714BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum D-amino acid dehydrogenase small subunit (dadA) CSB-YP806715BVW
CSB-EP806715BVW
CSB-BP806715BVW
CSB-MP806715BVW
CSB-EP806715BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum UPF0337 protein blr1496 (blr1496) CSB-YP806716BVW
CSB-EP806716BVW
CSB-BP806716BVW
CSB-MP806716BVW
CSB-EP806716BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Urease subunit gamma (ureA) CSB-YP806717BVW
CSB-EP806717BVW
CSB-BP806717BVW
CSB-MP806717BVW
CSB-EP806717BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1 (ispH1) CSB-YP806718BVW
CSB-EP806718BVW
CSB-BP806718BVW
CSB-MP806718BVW
CSB-EP806718BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Xylose isomerase (xylA) CSB-YP806719BVW
CSB-EP806719BVW
CSB-BP806719BVW
CSB-MP806719BVW
CSB-EP806719BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP806720BVW
CSB-EP806720BVW
CSB-BP806720BVW
CSB-MP806720BVW
CSB-EP806720BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Putative phosphoribosylaminoimidazole-succinocarboxamide synthase B (purC2) CSB-YP806721BVW
CSB-EP806721BVW
CSB-BP806721BVW
CSB-MP806721BVW
CSB-EP806721BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 30S ribosomal protein S15 (rpsO) CSB-YP806722BVW
CSB-EP806722BVW
CSB-BP806722BVW
CSB-MP806722BVW
CSB-EP806722BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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