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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP807703WAN
CSB-EP807703WAN
CSB-BP807703WAN
CSB-MP807703WAN
CSB-EP807703WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD) CSB-YP807704WAN
CSB-EP807704WAN
CSB-BP807704WAN
CSB-MP807704WAN
CSB-EP807704WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP807705WAN
CSB-EP807705WAN
CSB-BP807705WAN
CSB-MP807705WAN
CSB-EP807705WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L35 (rpmI) CSB-YP807706WAN
CSB-EP807706WAN
CSB-BP807706WAN
CSB-MP807706WAN
CSB-EP807706WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease H (rnhA) CSB-YP807707WAN
CSB-EP807707WAN
CSB-BP807707WAN
CSB-MP807707WAN
CSB-EP807707WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor G (fusA), partial CSB-YP807708WAN
CSB-EP807708WAN
CSB-BP807708WAN
CSB-MP807708WAN
CSB-EP807708WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA modification GTPase MnmE (mnmE) CSB-YP807709WAN
CSB-EP807709WAN
CSB-BP807709WAN
CSB-MP807709WAN
CSB-EP807709WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit alpha (atpA), partial CSB-YP807710WAN
CSB-EP807710WAN
CSB-BP807710WAN
CSB-MP807710WAN
CSB-EP807710WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphonoacetaldehyde hydrolase (phnX) CSB-YP807711VFI
CSB-EP807711VFI
CSB-BP807711VFI
CSB-MP807711VFI
CSB-EP807711VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maltose/maltodextrin import ATP-binding protein MalK (malK) CSB-YP807712VFI
CSB-EP807712VFI
CSB-BP807712VFI
CSB-MP807712VFI
CSB-EP807712VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaerobic nitric oxide reductase transcription regulator norR (norR), partial CSB-YP807713VFI
CSB-EP807713VFI
CSB-BP807713VFI
CSB-MP807713VFI
CSB-EP807713VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucosamine-6-phosphate deaminase (nagB) CSB-YP807714VFI
CSB-EP807714VFI
CSB-BP807714VFI
CSB-MP807714VFI
CSB-EP807714VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tyrosine--tRNA ligase 1 (tyrS1) CSB-YP807715VFI
CSB-EP807715VFI
CSB-BP807715VFI
CSB-MP807715VFI
CSB-EP807715VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant H (+)/Cl (-) exchange transporter ClcA (clcA), partial CSB-YP807716VFI
CSB-EP807716VFI
CSB-BP807716VFI
CSB-MP807716VFI
CSB-EP807716VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-oxoacyl-[acyl-carrier-protein] synthase 3 protein 2 (fabH2) CSB-YP807717VFI
CSB-EP807717VFI
CSB-BP807717VFI
CSB-MP807717VFI
CSB-EP807717VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative reductase VV2_0265 (VV2_0265) CSB-YP807718VFI
CSB-EP807718VFI
CSB-BP807718VFI
CSB-MP807718VFI
CSB-EP807718VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribose import ATP-binding protein RbsA (rbsA), partial CSB-YP807719VFI
CSB-EP807719VFI
CSB-BP807719VFI
CSB-MP807719VFI
CSB-EP807719VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Electron transport complex protein RnfA (rnfA), partial CSB-YP807720VFI1
CSB-EP807720VFI1
CSB-BP807720VFI1
CSB-MP807720VFI1
CSB-EP807720VFI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0263 protein VV1_3059 (VV1_3059) CSB-YP807721VFI
CSB-EP807721VFI
CSB-BP807721VFI
CSB-MP807721VFI
CSB-EP807721VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphate acyltransferase (plsX) CSB-YP807722VFI
CSB-EP807722VFI
CSB-BP807722VFI
CSB-MP807722VFI
CSB-EP807722VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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