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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bradyrhizobium japonicum Glycerol-3-phosphate dehydrogenase [NAD (P)+] (gpsA) CSB-YP807243BVW
CSB-EP807243BVW
CSB-BP807243BVW
CSB-MP807243BVW
CSB-EP807243BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 3-isopropylmalate dehydrogenase 2 (leuB2) CSB-YP807244BVW
CSB-EP807244BVW
CSB-BP807244BVW
CSB-MP807244BVW
CSB-EP807244BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Diaminopimelate epimerase (dapF) CSB-YP807245BVW
CSB-EP807245BVW
CSB-BP807245BVW
CSB-MP807245BVW
CSB-EP807245BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum ATP synthase gamma chain (atpG) CSB-YP807246BVW
CSB-EP807246BVW
CSB-BP807246BVW
CSB-MP807246BVW
CSB-EP807246BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Glutamate 5-kinase (proB) CSB-YP807247BVW
CSB-EP807247BVW
CSB-BP807247BVW
CSB-MP807247BVW
CSB-EP807247BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 1-aminocyclopropane-1-carboxylate deaminase (acdS) CSB-YP807248BVW
CSB-EP807248BVW
CSB-BP807248BVW
CSB-MP807248BVW
CSB-EP807248BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Protein translocase subunit SecA (secA), partial CSB-YP807249BVW
CSB-EP807249BVW
CSB-BP807249BVW
CSB-MP807249BVW
CSB-EP807249BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Probable endonuclease 4 (nfo) CSB-YP807250BDU
CSB-EP807250BDU
CSB-BP807250BDU
CSB-MP807250BDU
CSB-EP807250BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Protein CrcB homolog (crcB), partial CSB-YP807251BDU1
CSB-EP807251BDU1
CSB-BP807251BDU1
CSB-MP807251BDU1
CSB-EP807251BDU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Copper homeostasis protein CutC (cutC) CSB-YP807252BDU
CSB-EP807252BDU
CSB-BP807252BDU
CSB-MP807252BDU
CSB-EP807252BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron tRNA dimethylallyltransferase 2 (miaA2) CSB-YP807253BDU
CSB-EP807253BDU
CSB-BP807253BDU
CSB-MP807253BDU
CSB-EP807253BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron tRNA pseudouridine synthase A (truA) CSB-YP807254BDU
CSB-EP807254BDU
CSB-BP807254BDU
CSB-MP807254BDU
CSB-EP807254BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP807255BDU
CSB-EP807255BDU
CSB-BP807255BDU
CSB-MP807255BDU
CSB-EP807255BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP807256BDU
CSB-EP807256BDU
CSB-BP807256BDU
CSB-MP807256BDU
CSB-EP807256BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Elongation factor Ts (tsf) CSB-YP807257BDU
CSB-EP807257BDU
CSB-BP807257BDU
CSB-MP807257BDU
CSB-EP807257BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron GTPase Der (der) CSB-YP807258BDU
CSB-EP807258BDU
CSB-BP807258BDU
CSB-MP807258BDU
CSB-EP807258BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Undecaprenyl pyrophosphate synthase (uppS) CSB-YP807259BDU
CSB-EP807259BDU
CSB-BP807259BDU
CSB-MP807259BDU
CSB-EP807259BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Acetate kinase (ackA) CSB-YP807260BDU
CSB-EP807260BDU
CSB-BP807260BDU
CSB-MP807260BDU
CSB-EP807260BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP807261BDU
CSB-EP807261BDU
CSB-BP807261BDU
CSB-MP807261BDU
CSB-EP807261BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Putative K (+)-stimulated pyrophosphate-energized sodium pump (hppA), partial CSB-YP807262BDU
CSB-EP807262BDU
CSB-BP807262BDU
CSB-MP807262BDU
CSB-EP807262BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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