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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Mouse Actin-binding LIM protein 1 (Ablim1), partial CSB-YP809113MO
CSB-EP809113MO
CSB-BP809113MO
CSB-MP809113MO
CSB-EP809113MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat BPI fold-containing family A member 1 (Bpifa1) CSB-YP809114RA
CSB-EP809114RA
CSB-BP809114RA
CSB-MP809114RA
CSB-EP809114RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse MKL/myocardin-like protein 1 (Mkl1), partial CSB-YP809115MO
CSB-EP809115MO
CSB-BP809115MO
CSB-MP809115MO
CSB-EP809115MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Actin-binding Rho-activating protein (Abra) CSB-YP809116RA
CSB-EP809116RA
CSB-BP809116RA
CSB-MP809116RA
CSB-EP809116RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Beta-defensin 12 (Defb12) CSB-YP809117MO
CSB-EP809117MO
CSB-BP809117MO
CSB-MP809117MO
CSB-EP809117MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Endonuclease 8-like 1 (Neil1) CSB-YP809118MO
CSB-EP809118MO
CSB-BP809118MO
CSB-MP809118MO
CSB-EP809118MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Cytoplasmic phosphatidylinositol transfer protein 1 (Pitpnc1) CSB-YP809119MO
CSB-EP809119MO
CSB-BP809119MO
CSB-MP809119MO
CSB-EP809119MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Filamin-A-interacting protein 1 (Filip1), partial CSB-YP809120RA
CSB-EP809120RA
CSB-BP809120RA
CSB-MP809120RA
CSB-EP809120RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Leucine-rich repeat LGI family member 2 (Lgi2) CSB-YP809121MO
CSB-EP809121MO
CSB-BP809121MO
CSB-MP809121MO
CSB-EP809121MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat tRNA-dihydrouridine (16/17) synthase [NAD (P) (+)]-like (Dus1l) CSB-YP809122RA
CSB-EP809122RA
CSB-BP809122RA
CSB-MP809122RA
CSB-EP809122RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Multiple coagulation factor deficiency protein 2 homolog (Mcfd2) CSB-YP809123MO
CSB-EP809123MO
CSB-BP809123MO
CSB-MP809123MO
CSB-EP809123MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Uncharacterized transporter BUsg_567 (BUsg_567), partial CSB-YP809124BXE
CSB-EP809124BXE
CSB-BP809124BXE
CSB-MP809124BXE
CSB-EP809124BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Putative N-acetylmuramoyl-L-alanine amidase (amiB) CSB-YP809125BXE
CSB-EP809125BXE
CSB-BP809125BXE
CSB-MP809125BXE
CSB-EP809125BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP809126BXE
CSB-EP809126BXE
CSB-BP809126BXE
CSB-MP809126BXE
CSB-EP809126BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum tRNA (guanine-N (7)-)-methyltransferase (trmB) CSB-YP809127BXE
CSB-EP809127BXE
CSB-BP809127BXE
CSB-MP809127BXE
CSB-EP809127BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Single-stranded DNA-binding protein (ssb) CSB-YP809128BXE
CSB-EP809128BXE
CSB-BP809128BXE
CSB-MP809128BXE
CSB-EP809128BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Phosphopentomutase (deoB) CSB-YP809129BXE
CSB-EP809129BXE
CSB-BP809129BXE
CSB-MP809129BXE
CSB-EP809129BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum FKBP-type peptidyl-prolyl cis-trans isomerase fkpA (fkpA) CSB-YP809130BXE
CSB-EP809130BXE
CSB-BP809130BXE
CSB-MP809130BXE
CSB-EP809130BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum 50S ribosomal protein L3 (rplC) CSB-YP809131BXE
CSB-EP809131BXE
CSB-BP809131BXE
CSB-MP809131BXE
CSB-EP809131BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum 50S ribosomal protein L5 (rplE) CSB-YP809132BXE
CSB-EP809132BXE
CSB-BP809132BXE
CSB-MP809132BXE
CSB-EP809132BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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