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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Flagellar motor switch protein FliN (fliN) CSB-YP807183BMZ
CSB-EP807183BMZ
CSB-BP807183BMZ
CSB-MP807183BMZ
CSB-EP807183BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Phosphocarrier protein HPr (ptsH) CSB-YP807184BMZ
CSB-EP807184BMZ
CSB-BP807184BMZ
CSB-MP807184BMZ
CSB-EP807184BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 50S ribosomal protein L11 (rplK) CSB-YP807185BMZ
CSB-EP807185BMZ
CSB-BP807185BMZ
CSB-MP807185BMZ
CSB-EP807185BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae DNA-binding protein HU (hup) CSB-YP807186BMZ
CSB-EP807186BMZ
CSB-BP807186BMZ
CSB-MP807186BMZ
CSB-EP807186BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Ribosomal RNA small subunit methyltransferase D (rsmD) CSB-YP807187BMZ
CSB-EP807187BMZ
CSB-BP807187BMZ
CSB-MP807187BMZ
CSB-EP807187BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 50S ribosomal protein L34 (rpmH) CSB-YP807188BMZ
CSB-EP807188BMZ
CSB-BP807188BMZ
CSB-MP807188BMZ
CSB-EP807188BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae ATP synthase gamma chain (atpG) CSB-YP807189BMZ
CSB-EP807189BMZ
CSB-BP807189BMZ
CSB-MP807189BMZ
CSB-EP807189BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Elongation factor 4 (lepA), partial CSB-YP807190BVW
CSB-EP807190BVW
CSB-BP807190BVW
CSB-MP807190BVW
CSB-EP807190BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Probable GTP-binding protein EngB (engB) CSB-YP807191BVW
CSB-EP807191BVW
CSB-BP807191BVW
CSB-MP807191BVW
CSB-EP807191BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Chaperone protein htpG (htpG), partial CSB-YP807192BVW
CSB-EP807192BVW
CSB-BP807192BVW
CSB-MP807192BVW
CSB-EP807192BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum DNA mismatch repair protein mutL (mutL), partial CSB-YP807193BVW
CSB-EP807193BVW
CSB-BP807193BVW
CSB-MP807193BVW
CSB-EP807193BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Peptidyl-tRNA hydrolase (pth) CSB-YP807194BVW
CSB-EP807194BVW
CSB-BP807194BVW
CSB-MP807194BVW
CSB-EP807194BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Uracil phosphoribosyltransferase (upp) CSB-YP807195BVW
CSB-EP807195BVW
CSB-BP807195BVW
CSB-MP807195BVW
CSB-EP807195BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP807196BVW
CSB-EP807196BVW
CSB-BP807196BVW
CSB-MP807196BVW
CSB-EP807196BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Flagellar motor switch protein FliG (fliG) CSB-YP807197BVW
CSB-EP807197BVW
CSB-BP807197BVW
CSB-MP807197BVW
CSB-EP807197BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Potassium-transporting ATPase C chain (kdpC), partial CSB-YP807198BVW
CSB-EP807198BVW
CSB-BP807198BVW
CSB-MP807198BVW
CSB-EP807198BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP807199BVW
CSB-EP807199BVW
CSB-BP807199BVW
CSB-MP807199BVW
CSB-EP807199BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum tRNA dimethylallyltransferase (miaA) CSB-YP807200BVW
CSB-EP807200BVW
CSB-BP807200BVW
CSB-MP807200BVW
CSB-EP807200BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 2-isopropylmalate synthase (leuA), partial CSB-YP807201BVW
CSB-EP807201BVW
CSB-BP807201BVW
CSB-MP807201BVW
CSB-EP807201BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Adenosylhomocysteinase (ahcY) CSB-YP807202BVW
CSB-EP807202BVW
CSB-BP807202BVW
CSB-MP807202BVW
CSB-EP807202BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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