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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptococcus gordonii 6-phosphofructokinase (pfkA) CSB-YP017822FMN
CSB-EP017822FMN
CSB-BP017822FMN
CSB-MP017822FMN
CSB-EP017822FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Giardia intestinalis Flap endonuclease 1 (FEN1) CSB-YP008585GFY
CSB-EP008585GFY
CSB-BP008585GFY
CSB-MP008585GFY
CSB-EP008585GFY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca cyclopis Agouti-signaling protein (ASIP) CSB-YP002212MMW
CSB-EP002212MMW
CSB-BP002212MMW
CSB-MP002212MMW
CSB-EP002212MMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca silenus Agouti-signaling protein (ASIP) CSB-YP002212MNC
CSB-EP002212MNC
CSB-BP002212MNC
CSB-MP002212MNC
CSB-EP002212MNC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avahi cleesei NADH-ubiquinone oxidoreductase chain 3 (MT-ND3), partial CSB-YP015078AVH1
CSB-EP015078AVH1
CSB-BP015078AVH1
CSB-MP015078AVH1
CSB-EP015078AVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein mab-21-like 3 (mab21L3) CSB-YP003543DIL
CSB-EP003543DIL
CSB-BP003543DIL
CSB-MP003543DIL
CSB-EP003543DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein FAM166B (fam166b) CSB-YP008121XBF
CSB-EP008121XBF
CSB-BP008121XBF
CSB-MP008121XBF
CSB-EP008121XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Pancreatic progenitor cell differentiation and proliferation factor-like protein CSB-YP004202BO
CSB-EP004202BO
CSB-BP004202BO
CSB-MP004202BO
CSB-EP004202BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Ribonuclease 3 (rnc) CSB-YP019809AUY
CSB-EP019809AUY
CSB-BP019809AUY
CSB-MP019809AUY
CSB-EP019809AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis ATP synthase subunit a (atpB), partial CSB-YP015070RMR1
CSB-EP015070RMR1
CSB-BP015070RMR1
CSB-MP015070RMR1
CSB-EP015070RMR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Elongation factor G (fusA), partial CSB-YP009373RMR
CSB-EP009373RMR
CSB-BP009373RMR
CSB-MP009373RMR
CSB-EP009373RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia massiliae Translation initiation factor IF-2 (infB), partial CSB-YP015164RIN
CSB-EP015164RIN
CSB-BP015164RIN
CSB-MP015164RIN
CSB-EP015164RIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga lettingae tRNA pseudouridine synthase A (truA) CSB-YP019086TKE
CSB-EP019086TKE
CSB-BP019086TKE
CSB-MP019086TKE
CSB-EP019086TKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824BOL
CSB-EP005824BOL
CSB-BP005824BOL
CSB-MP005824BOL
CSB-EP005824BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146BOL
CSB-EP019146BOL
CSB-BP019146BOL
CSB-MP019146BOL
CSB-EP019146BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Porphobilinogen deaminase (hemC) CSB-YP010524BOL
CSB-EP010524BOL
CSB-BP010524BOL
CSB-MP010524BOL
CSB-EP010524BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Triosephosphate isomerase (tpiA) CSB-YP024102BOL
CSB-EP024102BOL
CSB-BP024102BOL
CSB-MP024102BOL
CSB-EP024102BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:6 Elongation factor Ts (tsf) CSB-YP025124CWG
CSB-EP025124CWG
CSB-BP025124CWG
CSB-MP025124CWG
CSB-EP025124CWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Methionyl-tRNA formyltransferase (fmt) CSB-YP015150STR
CSB-EP015150STR
CSB-BP015150STR
CSB-MP015150STR
CSB-EP015150STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Malate dehydrogenase (mdh) CSB-YP013623STR
CSB-EP013623STR
CSB-BP013623STR
CSB-MP013623STR
CSB-EP013623STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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