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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rat Macrophage colony-stimulating factor 1 (Csf1), partial CSB-YP810353RA
CSB-BP810353RA
CSB-MP810353RA
CSB-EP810353RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse NEDD4-binding protein 2-like 2 (N4bp2l2) CSB-YP810354MO
CSB-EP810354MO
CSB-BP810354MO
CSB-MP810354MO
CSB-EP810354MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse SH2 domain-containing protein 5 (Sh2d5) CSB-YP810355MO
CSB-EP810355MO
CSB-BP810355MO
CSB-MP810355MO
CSB-EP810355MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Formin-binding protein 1-like (Fnbp1l) CSB-YP810356MO
CSB-EP810356MO
CSB-BP810356MO
CSB-MP810356MO
CSB-EP810356MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Serine/threonine-protein kinase N3 (Pkn3), partial CSB-YP810357MO
CSB-EP810357MO
CSB-BP810357MO
CSB-MP810357MO
CSB-EP810357MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Probable lysosomal cobalamin transporter (Lmbrd1), partial CSB-YP810358MO
CSB-EP810358MO
CSB-BP810358MO
CSB-MP810358MO
CSB-EP810358MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Cyclin-dependent kinase 17 (Cdk17) CSB-YP810359MO
CSB-EP810359MO
CSB-BP810359MO
CSB-MP810359MO
CSB-EP810359MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Rho GTPase-activating protein 18 (Arhgap18) CSB-YP810360MO
CSB-EP810360MO
CSB-BP810360MO
CSB-MP810360MO
CSB-EP810360MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse snRNA-activating protein complex subunit 1 (Snapc1) CSB-YP810361MO
CSB-EP810361MO
CSB-BP810361MO
CSB-MP810361MO
CSB-EP810361MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse DCN1-like protein 3 (Dcun1d3) CSB-YP810362MO
CSB-EP810362MO
CSB-BP810362MO
CSB-MP810362MO
CSB-EP810362MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Pleckstrin homology domain-containing family O member 2 (Plekho2) CSB-YP810363MO
CSB-EP810363MO
CSB-BP810363MO
CSB-MP810363MO
CSB-EP810363MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Keratinocyte-associated protein 3 (Krtcap3), partial CSB-YP810364MO1
CSB-EP810364MO1
CSB-BP810364MO1
CSB-MP810364MO1
CSB-EP810364MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Fermitin family homolog 3 (Fermt3) CSB-YP810365MO
CSB-EP810365MO
CSB-BP810365MO
CSB-MP810365MO
CSB-EP810365MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 (Alkbh3) CSB-YP810366MO
CSB-EP810366MO
CSB-BP810366MO
CSB-MP810366MO
CSB-EP810366MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse UPF0667 protein C1orf55 homolog CSB-YP810367MO
CSB-EP810367MO
CSB-BP810367MO
CSB-MP810367MO
CSB-EP810367MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein phosphatase 1 regulatory subunit 11 (Ppp1r11) CSB-YP810368MO
CSB-EP810368MO
CSB-BP810368MO
CSB-MP810368MO
CSB-EP810368MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Pleckstrin homology-like domain family B member 2 (Phldb2), partial CSB-YP810369MO
CSB-EP810369MO
CSB-BP810369MO
CSB-MP810369MO
CSB-EP810369MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Serine/threonine-protein kinase Nek9 (Nek9), partial CSB-YP810370MO
CSB-EP810370MO
CSB-BP810370MO
CSB-MP810370MO
CSB-EP810370MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Transmembrane protease serine 3 (Tmprss3), partial CSB-YP810371MO
CSB-EP810371MO
CSB-BP810371MO
CSB-MP810371MO
CSB-EP810371MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse N-arachidonyl glycine receptor (Gpr18), partial CSB-YP810372MO1
CSB-EP810372MO1
CSB-BP810372MO1
CSB-MP810372MO1
CSB-EP810372MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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