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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Danio rerio Thrombospondin-4-B (thbs4b), partial CSB-YP810333DIL
CSB-EP810333DIL
CSB-BP810333DIL
CSB-MP810333DIL
CSB-EP810333DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Fructose-bisphosphate aldolase C-B (aldocb) CSB-YP810334DIL
CSB-EP810334DIL
CSB-BP810334DIL
CSB-MP810334DIL
CSB-EP810334DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein lin-28 homolog A (lin28a) CSB-YP810335XBE
CSB-EP810335XBE
CSB-BP810335XBE
CSB-MP810335XBE
CSB-EP810335XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe (lfng), partial CSB-YP810336DIL
CSB-EP810336DIL
CSB-BP810336DIL
CSB-MP810336DIL
CSB-EP810336DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio ATP-dependent RNA helicase DDX55 (ddx55), partial CSB-YP810337DIL
CSB-EP810337DIL
CSB-BP810337DIL
CSB-MP810337DIL
CSB-EP810337DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Paladin (PALD), partial CSB-YP810338CH
CSB-EP810338CH
CSB-BP810338CH
CSB-MP810338CH
CSB-EP810338CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken D-erythrulose reductase (DER) CSB-YP810339CH
CSB-EP810339CH
CSB-BP810339CH
CSB-MP810339CH
CSB-EP810339CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protobothrops jerdonii Phospholipase A2 jerdoxin CSB-YP810340EZC
CSB-EP810340EZC
CSB-BP810340EZC
CSB-MP810340EZC
CSB-EP810340EZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Zinc finger protein ZIC 1 (ZIC1) CSB-YP810341CH
CSB-EP810341CH
CSB-BP810341CH
CSB-MP810341CH
CSB-EP810341CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ectromelia virus Profilin (EVM141) CSB-YP810342EDAD
CSB-EP810342EDAD
CSB-BP810342EDAD
CSB-MP810342EDAD
CSB-EP810342EDAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ectromelia virus DNA-directed RNA polymerase 132 kDa polypeptide (RPO132), partial CSB-YP810343EDAD
CSB-EP810343EDAD
CSB-BP810343EDAD
CSB-MP810343EDAD
CSB-EP810343EDAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pear latent virus RNA silencing suppressor p19 (ORF4) CSB-YP810344PJAE
CSB-EP810344PJAE
CSB-BP810344PJAE
CSB-MP810344PJAE
CSB-EP810344PJAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hepatitis B virus genotype H Protein P (P), partial CSB-YP810345HEAR
CSB-EP810345HEAR
CSB-BP810345HEAR
CSB-MP810345HEAR
CSB-EP810345HEAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine enteric calicivirus NB Genome polyprotein (ORF1), partial CSB-YP810346BEAK
CSB-EP810346BEAK
CSB-BP810346BEAK
CSB-MP810346BEAK
CSB-EP810346BEAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Snake adenovirus serotype 1 Hexon protein (PII), partial CSB-YP810347SIAF
CSB-EP810347SIAF
CSB-BP810347SIAF
CSB-MP810347SIAF
CSB-EP810347SIAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Reston ebolavirus Matrix protein VP40 (VP40) CSB-YP810348RCJ
CSB-BP810348RCJ
CSB-MP810348RCJ
CSB-EP810348RCJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crimean-Congo hemorrhagic fever virus Envelope glycoprotein (GP), partial CSB-YP810349CSC1
CSB-EP810349CSC1
CSB-BP810349CSC1
CSB-MP810349CSC1
CSB-EP810349CSC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquareovirus C Non-structural protein 3 (S11) CSB-YP810350AHAG
CSB-EP810350AHAG
CSB-BP810350AHAG
CSB-MP810350AHAG
CSB-EP810350AHAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chikungunya virus Non-structural polyprotein, partial CSB-YP810351CJAT
CSB-BP810351CJAT
CSB-MP810351CJAT
CSB-EP810351CJAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Proline-rich protein 15-like protein (Prr15l) CSB-YP810352MO
CSB-EP810352MO
CSB-BP810352MO
CSB-MP810352MO
CSB-EP810352MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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