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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant 30S ribosomal protein S20 (rpsT) CSB-YP810763WAN
CSB-EP810763WAN
CSB-BP810763WAN
CSB-MP810763WAN
CSB-EP810763WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent protease subunit HslV (hslV) CSB-YP810764WAN
CSB-EP810764WAN
CSB-BP810764WAN
CSB-MP810764WAN
CSB-EP810764WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L19 (rplS) CSB-YP810765WAN
CSB-EP810765WAN
CSB-BP810765WAN
CSB-MP810765WAN
CSB-EP810765WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cysteine--tRNA ligase (cysS) CSB-YP810766WAN
CSB-EP810766WAN
CSB-BP810766WAN
CSB-MP810766WAN
CSB-EP810766WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP810767WAN
CSB-EP810767WAN
CSB-BP810767WAN
CSB-MP810767WAN
CSB-EP810767WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP810768WAN
CSB-EP810768WAN
CSB-BP810768WAN
CSB-MP810768WAN
CSB-EP810768WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP810769WAN
CSB-EP810769WAN
CSB-BP810769WAN
CSB-MP810769WAN
CSB-EP810769WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP810770WAN
CSB-EP810770WAN
CSB-BP810770WAN
CSB-MP810770WAN
CSB-EP810770WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative Holliday junction resolvase (WIGBR1640) CSB-YP810771WAN
CSB-EP810771WAN
CSB-BP810771WAN
CSB-MP810771WAN
CSB-EP810771WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protoheme IX farnesyltransferase (cyoE), partial CSB-YP810772WAN
CSB-EP810772WAN
CSB-BP810772WAN
CSB-MP810772WAN
CSB-EP810772WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP810773WAN
CSB-EP810773WAN
CSB-BP810773WAN
CSB-MP810773WAN
CSB-EP810773WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thymidylate kinase (tmk) CSB-YP810774WAN
CSB-EP810774WAN
CSB-BP810774WAN
CSB-MP810774WAN
CSB-EP810774WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Threonine--tRNA ligase (thrS), partial CSB-YP810775WAN
CSB-EP810775WAN
CSB-BP810775WAN
CSB-MP810775WAN
CSB-EP810775WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucans biosynthesis glucosyltransferase H (mdoH), partial CSB-YP810776WAN
CSB-EP810776WAN
CSB-BP810776WAN
CSB-MP810776WAN
CSB-EP810776WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP810777WAN
CSB-EP810777WAN
CSB-BP810777WAN
CSB-MP810777WAN
CSB-EP810777WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS), partial CSB-YP810778WAN
CSB-EP810778WAN
CSB-BP810778WAN
CSB-MP810778WAN
CSB-EP810778WAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit c (atpE), partial CSB-YP810779WAN1
CSB-EP810779WAN1
CSB-BP810779WAN1
CSB-MP810779WAN1
CSB-EP810779WAN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hemin import ATP-binding protein HmuV (hmuV) CSB-YP810780VFI
CSB-EP810780VFI
CSB-BP810780VFI
CSB-MP810780VFI
CSB-EP810780VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-threonine 3-dehydrogenase (tdh) CSB-YP810781VFI
CSB-EP810781VFI
CSB-BP810781VFI
CSB-MP810781VFI
CSB-EP810781VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitric oxide reductase FlRd-NAD (+) reductase (norW) CSB-YP810782VFI
CSB-EP810782VFI
CSB-BP810782VFI
CSB-MP810782VFI
CSB-EP810782VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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