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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Thermosynechococcus elongatus Shikimate dehydrogenase (aroE) CSB-YP812166TDQ
CSB-EP812166TDQ
CSB-BP812166TDQ
CSB-MP812166TDQ
CSB-EP812166TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP812167TDQ
CSB-EP812167TDQ
CSB-BP812167TDQ
CSB-MP812167TDQ
CSB-EP812167TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP812168TDQ
CSB-EP812168TDQ
CSB-BP812168TDQ
CSB-MP812168TDQ
CSB-EP812168TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP812169TDQ
CSB-EP812169TDQ
CSB-BP812169TDQ
CSB-MP812169TDQ
CSB-EP812169TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus ATP synthase gamma chain (atpG) CSB-YP812171TDQ
CSB-EP812171TDQ
CSB-BP812171TDQ
CSB-MP812171TDQ
CSB-EP812171TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Ribosomal protein L11 methyltransferase (prmA) CSB-YP812172TDQ
CSB-EP812172TDQ
CSB-BP812172TDQ
CSB-MP812172TDQ
CSB-EP812172TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP812173TDQ
CSB-EP812173TDQ
CSB-BP812173TDQ
CSB-MP812173TDQ
CSB-EP812173TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA) CSB-YP812174TDQ
CSB-EP812174TDQ
CSB-BP812174TDQ
CSB-MP812174TDQ
CSB-EP812174TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 50S ribosomal protein L21 (rplU) CSB-YP812175TDQ
CSB-EP812175TDQ
CSB-BP812175TDQ
CSB-MP812175TDQ
CSB-EP812175TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Translation initiation factor IF-1 (infA) CSB-YP812176TDQ
CSB-EP812176TDQ
CSB-BP812176TDQ
CSB-MP812176TDQ
CSB-EP812176TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 50S ribosomal protein L5 (rplE) CSB-YP812177TDQ
CSB-EP812177TDQ
CSB-BP812177TDQ
CSB-MP812177TDQ
CSB-EP812177TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 50S ribosomal protein L22 (rplV) CSB-YP812178TDQ
CSB-EP812178TDQ
CSB-BP812178TDQ
CSB-MP812178TDQ
CSB-EP812178TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Urease accessory protein UreG (ureG) CSB-YP812179TDQ
CSB-EP812179TDQ
CSB-BP812179TDQ
CSB-MP812179TDQ
CSB-EP812179TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Urease subunit alpha (ureC), partial CSB-YP812180TDQ
CSB-EP812180TDQ
CSB-BP812180TDQ
CSB-MP812180TDQ
CSB-EP812180TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (dapH) CSB-YP812181SMP
CSB-EP812181SMP
CSB-BP812181SMP
CSB-MP812181SMP
CSB-EP812181SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Membrane protein insertase YidC 1 (yidC1), partial CSB-YP812182SMP1
CSB-EP812182SMP1
CSB-BP812182SMP1
CSB-MP812182SMP1
CSB-EP812182SMP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae UPF0374 protein spr1719 (spr1719) CSB-YP812183SMP
CSB-EP812183SMP
CSB-BP812183SMP
CSB-MP812183SMP
CSB-EP812183SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Indole-3-glycerol phosphate synthase (trpC) CSB-YP812184SMP
CSB-EP812184SMP
CSB-BP812184SMP
CSB-MP812184SMP
CSB-EP812184SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae UPF0348 protein spr1587 (spr1587) CSB-YP812185SMP
CSB-EP812185SMP
CSB-BP812185SMP
CSB-MP812185SMP
CSB-EP812185SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae D-tyrosyl-tRNA (Tyr) deacylase (dtd) CSB-YP812186SMP
CSB-EP812186SMP
CSB-BP812186SMP
CSB-MP812186SMP
CSB-EP812186SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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