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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP811363CQG
CSB-EP811363CQG
CSB-BP811363CQG
CSB-MP811363CQG
CSB-EP811363CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L35 (rpmI) CSB-YP811364CQG
CSB-EP811364CQG
CSB-BP811364CQG
CSB-MP811364CQG
CSB-EP811364CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP811365CQG
CSB-EP811365CQG
CSB-BP811365CQG
CSB-MP811365CQG
CSB-EP811365CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromosomal replication initiator protein DnaA (dnaA), partial CSB-YP811366CQG
CSB-EP811366CQG
CSB-BP811366CQG
CSB-MP811366CQG
CSB-EP811366CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Flagellar M-ring protein FliF (fliF), partial CSB-YP811367BMQ1
CSB-EP811367BMQ1
CSB-BP811367BMQ1
CSB-MP811367BMQ1
CSB-EP811367BMQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Zinc import ATP-binding protein ZnuC (znuC) CSB-YP811368BMQ
CSB-EP811368BMQ
CSB-BP811368BMQ
CSB-MP811368BMQ
CSB-EP811368BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP811369BMQ
CSB-EP811369BMQ
CSB-BP811369BMQ
CSB-MP811369BMQ
CSB-EP811369BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Ornithine cyclodeaminase (arcB) CSB-YP811370BMQ
CSB-EP811370BMQ
CSB-BP811370BMQ
CSB-MP811370BMQ
CSB-EP811370BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP811371BMQ
CSB-EP811371BMQ
CSB-BP811371BMQ
CSB-MP811371BMQ
CSB-EP811371BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Bifunctional protein FolD (folD) CSB-YP811372BMQ
CSB-EP811372BMQ
CSB-BP811372BMQ
CSB-MP811372BMQ
CSB-EP811372BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Alkyl hydroperoxide reductase AhpD (ahpD) CSB-YP811373BMQ
CSB-EP811373BMQ
CSB-BP811373BMQ
CSB-MP811373BMQ
CSB-EP811373BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Putative Holliday junction resolvase (BRA0597, BS1330_II0592) CSB-YP811374BMQ
CSB-EP811374BMQ
CSB-BP811374BMQ
CSB-MP811374BMQ
CSB-EP811374BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Bifunctional protein GlmU (glmU) CSB-YP811375BMQ
CSB-EP811375BMQ
CSB-BP811375BMQ
CSB-MP811375BMQ
CSB-EP811375BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP811376BMQ
CSB-EP811376BMQ
CSB-BP811376BMQ
CSB-MP811376BMQ
CSB-EP811376BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Leu/Ile/Val-binding protein homolog 8 (BRA0392, BS1330_II0389) CSB-YP811377BMQ
CSB-EP811377BMQ
CSB-BP811377BMQ
CSB-MP811377BMQ
CSB-EP811377BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Probable septum site-determining protein MinC (minC) CSB-YP811378BMQ
CSB-EP811378BMQ
CSB-BP811378BMQ
CSB-MP811378BMQ
CSB-EP811378BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Type IV secretion system protein VirB11 (virB11) CSB-YP811379BMQ
CSB-EP811379BMQ
CSB-BP811379BMQ
CSB-MP811379BMQ
CSB-EP811379BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP811380BMQ
CSB-EP811380BMQ
CSB-BP811380BMQ
CSB-MP811380BMQ
CSB-EP811380BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP811381BMQ
CSB-EP811381BMQ
CSB-BP811381BMQ
CSB-MP811381BMQ
CSB-EP811381BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Shikimate kinase (aroK) CSB-YP811382BMQ
CSB-EP811382BMQ
CSB-BP811382BMQ
CSB-MP811382BMQ
CSB-EP811382BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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