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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP810863VFI
CSB-EP810863VFI
CSB-BP810863VFI
CSB-MP810863VFI
CSB-EP810863VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Putative ABC transporter ATP-binding protein tll2439 (tll2439) CSB-YP810864TDQ
CSB-EP810864TDQ
CSB-BP810864TDQ
CSB-MP810864TDQ
CSB-EP810864TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP810865TDQ
CSB-EP810865TDQ
CSB-BP810865TDQ
CSB-MP810865TDQ
CSB-EP810865TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Light-independent protochlorophyllide reductase subunit N (chlN) CSB-YP810866TDQ
CSB-EP810866TDQ
CSB-BP810866TDQ
CSB-MP810866TDQ
CSB-EP810866TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Diaminopimelate epimerase (dapF) CSB-YP810867TDQ
CSB-EP810867TDQ
CSB-BP810867TDQ
CSB-MP810867TDQ
CSB-EP810867TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Phosphoglycerate kinase (pgk) CSB-YP810868TDQ
CSB-EP810868TDQ
CSB-BP810868TDQ
CSB-MP810868TDQ
CSB-EP810868TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Recombination protein RecR (recR) CSB-YP810869TDQ
CSB-EP810869TDQ
CSB-BP810869TDQ
CSB-MP810869TDQ
CSB-EP810869TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus D,D-heptose 1,7-bisphosphate phosphatase (gmhB) CSB-YP810870TDQ
CSB-EP810870TDQ
CSB-BP810870TDQ
CSB-MP810870TDQ
CSB-EP810870TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 30S ribosomal protein S18 (rpsR) CSB-YP810871TDQ
CSB-EP810871TDQ
CSB-BP810871TDQ
CSB-MP810871TDQ
CSB-EP810871TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus UPF0235 protein tsr1994 (tsr1994) CSB-YP810872TDQ
CSB-EP810872TDQ
CSB-BP810872TDQ
CSB-MP810872TDQ
CSB-EP810872TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Arginine biosynthesis bifunctional protein ArgJ (argJ) CSB-YP810873TDQ
CSB-EP810873TDQ
CSB-BP810873TDQ
CSB-MP810873TDQ
CSB-EP810873TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Release factor glutamine methyltransferase (prmC) CSB-YP810874TDQ
CSB-EP810874TDQ
CSB-BP810874TDQ
CSB-MP810874TDQ
CSB-EP810874TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP810875TDQ
CSB-EP810875TDQ
CSB-BP810875TDQ
CSB-MP810875TDQ
CSB-EP810875TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 5'-nucleotidase surE (surE) CSB-YP810876TDQ
CSB-EP810876TDQ
CSB-BP810876TDQ
CSB-MP810876TDQ
CSB-EP810876TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Elongation factor Tu (tuf) CSB-YP810877TDQ
CSB-EP810877TDQ
CSB-BP810877TDQ
CSB-MP810877TDQ
CSB-EP810877TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus UPF0758 protein tlr1707 (tlr1707) CSB-YP810878TDQ
CSB-EP810878TDQ
CSB-BP810878TDQ
CSB-MP810878TDQ
CSB-EP810878TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus 30S ribosomal protein S2 (rpsB) CSB-YP810879TDQ
CSB-EP810879TDQ
CSB-BP810879TDQ
CSB-MP810879TDQ
CSB-EP810879TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Porphobilinogen deaminase (hemC) CSB-YP810880TDQ
CSB-EP810880TDQ
CSB-BP810880TDQ
CSB-MP810880TDQ
CSB-EP810880TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP810881TDQ
CSB-EP810881TDQ
CSB-BP810881TDQ
CSB-MP810881TDQ
CSB-EP810881TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Photosystem II reaction center protein T (psbT), partial CSB-YP810882TDQ
CSB-EP810882TDQ
CSB-BP810882TDQ
CSB-MP810882TDQ
CSB-EP810882TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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