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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Swarming motility regulation protein rssB (rssB) CSB-YP811463SYN
CSB-EP811463SYN
CSB-BP811463SYN
CSB-MP811463SYN
CSB-EP811463SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodovulum sulfidophilum Dimethylsulfide dehydrogenase subunit beta (ddhB) CSB-YP811464RMF
CSB-EP811464RMF
CSB-BP811464RMF
CSB-MP811464RMF
CSB-EP811464RMF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Signal transduction protein TRAP (traP) CSB-YP811465FLL
CSB-EP811465FLL
CSB-BP811465FLL
CSB-MP811465FLL
CSB-EP811465FLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent protease subunit HslV (hslV) CSB-YP811466LBAZ
CSB-EP811466LBAZ
CSB-BP811466LBAZ
CSB-MP811466LBAZ
CSB-EP811466LBAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Homeobox-leucine zipper protein HOX19 (HOX19) CSB-YP811467OFG
CSB-EP811467OFG
CSB-BP811467OFG
CSB-MP811467OFG
CSB-EP811467OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prunus dulcis Profilin CSB-YP811468EZJ
CSB-EP811468EZJ
CSB-BP811468EZJ
CSB-MP811468EZJ
CSB-EP811468EZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana NEP1-interacting protein 2 (NIP2), partial CSB-YP811469DOA1
CSB-EP811469DOA1
CSB-BP811469DOA1
CSB-MP811469DOA1
CSB-EP811469DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Elongation factor 1-alpha 4 (A4) CSB-YP811470DOA
CSB-EP811470DOA
CSB-BP811470DOA
CSB-MP811470DOA
CSB-EP811470DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Pleiotropic drug resistance protein 4 (PDR4), partial CSB-YP811471OFG
CSB-EP811471OFG
CSB-BP811471OFG
CSB-MP811471OFG
CSB-EP811471OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized GPI-anchored protein At1g61900 (At1g61900) CSB-YP811472DOA
CSB-EP811472DOA
CSB-BP811472DOA
CSB-MP811472DOA
CSB-EP811472DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Galanthus nivalis Maturase K (matK), partial CSB-YP811473GAC
CSB-EP811473GAC
CSB-BP811473GAC
CSB-MP811473GAC
CSB-EP811473GAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana CAAX prenyl protease 2 (FACE2), partial CSB-YP811474DOA1
CSB-EP811474DOA1
CSB-BP811474DOA1
CSB-MP811474DOA1
CSB-EP811474DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Alpha-L-fucosidase 1 (FUC1) CSB-YP811475DOA
CSB-EP811475DOA
CSB-BP811475DOA
CSB-MP811475DOA
CSB-EP811475DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana CASP-like protein At2g39530 (At2g39530), partial CSB-YP811476DOA1
CSB-EP811476DOA1
CSB-BP811476DOA1
CSB-MP811476DOA1
CSB-EP811476DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cysteine-rich receptor-like protein kinase 19 (CRK19), partial CSB-YP811477DOA
CSB-EP811477DOA
CSB-BP811477DOA
CSB-MP811477DOA
CSB-EP811477DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor MYB39 (MYB39) CSB-YP811478DOA
CSB-EP811478DOA
CSB-BP811478DOA
CSB-MP811478DOA
CSB-EP811478DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein SKIP34 (SKIP34) CSB-YP811479DOA
CSB-EP811479DOA
CSB-BP811479DOA
CSB-MP811479DOA
CSB-EP811479DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Formin-like protein 9 (FH9), partial CSB-YP811480DOA1
CSB-EP811480DOA1
CSB-BP811480DOA1
CSB-MP811480DOA1
CSB-EP811480DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At1g61340 (At1g61340) CSB-YP811481DOA
CSB-EP811481DOA
CSB-BP811481DOA
CSB-MP811481DOA
CSB-EP811481DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Chloride channel protein CLC-e (CLC-E), partial CSB-YP811482DOA
CSB-EP811482DOA
CSB-BP811482DOA
CSB-MP811482DOA
CSB-EP811482DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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