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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Probable ATP-dependent helicase dinG (dinG) CSB-YP811303EGX
CSB-EP811303EGX
CSB-BP811303EGX
CSB-MP811303EGX
CSB-EP811303EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Quinolinate synthase A (nadA) CSB-YP811304EGX
CSB-EP811304EGX
CSB-BP811304EGX
CSB-MP811304EGX
CSB-EP811304EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apolipoprotein N-acyltransferase (lnt), partial CSB-YP811305EGX1
CSB-EP811305EGX1
CSB-BP811305EGX1
CSB-MP811305EGX1
CSB-EP811305EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Universal stress protein G (uspG) CSB-YP811306EGX
CSB-EP811306EGX
CSB-BP811306EGX
CSB-MP811306EGX
CSB-EP811306EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP811307EGX
CSB-EP811307EGX
CSB-BP811307EGX
CSB-MP811307EGX
CSB-EP811307EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adenylate kinase (adk) CSB-YP811308EGX
CSB-EP811308EGX
CSB-BP811308EGX
CSB-MP811308EGX
CSB-EP811308EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acyl carrier protein phosphodiesterase (acpH) CSB-YP811309EGX
CSB-EP811309EGX
CSB-BP811309EGX
CSB-MP811309EGX
CSB-EP811309EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Betaine aldehyde dehydrogenase (betB) CSB-YP811310EGX
CSB-EP811310EGX
CSB-BP811310EGX
CSB-MP811310EGX
CSB-EP811310EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease HII (rnhB) CSB-YP811311EGX
CSB-EP811311EGX
CSB-BP811311EGX
CSB-MP811311EGX
CSB-EP811311EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pantothenate synthetase (panC) CSB-YP811312EGX
CSB-EP811312EGX
CSB-BP811312EGX
CSB-MP811312EGX
CSB-EP811312EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP811313EGX
CSB-EP811313EGX
CSB-BP811313EGX
CSB-MP811313EGX
CSB-EP811313EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA polymerase-associated protein RapA (rapA), partial CSB-YP811314EGX
CSB-EP811314EGX
CSB-BP811314EGX
CSB-MP811314EGX
CSB-EP811314EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutathione-regulated potassium-efflux system protein kefC (kefC), partial CSB-YP811315EGX
CSB-EP811315EGX
CSB-BP811315EGX
CSB-MP811315EGX
CSB-EP811315EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carnitinyl-CoA dehydratase (caiD) CSB-YP811316EGX
CSB-EP811316EGX
CSB-BP811316EGX
CSB-MP811316EGX
CSB-EP811316EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tryptophan synthase beta chain 1 (trpB1) CSB-YP811317CQG
CSB-EP811317CQG
CSB-BP811317CQG
CSB-MP811317CQG
CSB-EP811317CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leucine--tRNA ligase (leuS), partial CSB-YP811318CQG
CSB-EP811318CQG
CSB-BP811318CQG
CSB-MP811318CQG
CSB-EP811318CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Membrane protein insertase YidC (yidC), partial CSB-YP811319CQG1
CSB-EP811319CQG1
CSB-BP811319CQG1
CSB-MP811319CQG1
CSB-EP811319CQG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoenolpyruvate carboxykinase [GTP] (pckG), partial CSB-YP811320CQG
CSB-EP811320CQG
CSB-BP811320CQG
CSB-MP811320CQG
CSB-EP811320CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carboxylate-amine ligase CE2574 (CE2574) CSB-YP811321CQG
CSB-EP811321CQG
CSB-BP811321CQG
CSB-MP811321CQG
CSB-EP811321CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA integrity scanning protein DisA (disA) CSB-YP811322CQG
CSB-EP811322CQG
CSB-BP811322CQG
CSB-MP811322CQG
CSB-EP811322CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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