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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brucella suis biovar 1 Porphobilinogen deaminase (hemC) CSB-YP811383BMQ
CSB-EP811383BMQ
CSB-BP811383BMQ
CSB-MP811383BMQ
CSB-EP811383BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 50S ribosomal protein L21 (rplU) CSB-YP811384BMQ
CSB-EP811384BMQ
CSB-BP811384BMQ
CSB-MP811384BMQ
CSB-EP811384BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 ATP synthase gamma chain (atpG) CSB-YP811385BMQ
CSB-EP811385BMQ
CSB-BP811385BMQ
CSB-MP811385BMQ
CSB-EP811385BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Thiamine-binding periplasmic protein (thiB) CSB-YP811386BMQ
CSB-EP811386BMQ
CSB-BP811386BMQ
CSB-MP811386BMQ
CSB-EP811386BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Elongation factor P (efp) CSB-YP811387BMQ
CSB-EP811387BMQ
CSB-BP811387BMQ
CSB-MP811387BMQ
CSB-EP811387BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 tRNA (Ile)-lysidine synthase (tilS) CSB-YP811388BMQ
CSB-EP811388BMQ
CSB-BP811388BMQ
CSB-MP811388BMQ
CSB-EP811388BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP811389BMQ
CSB-EP811389BMQ
CSB-BP811389BMQ
CSB-MP811389BMQ
CSB-EP811389BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 UPF0260 protein BR1477/BS1330_I1471 (BR1477, BS1330_I1471) CSB-YP811390BMQ
CSB-EP811390BMQ
CSB-BP811390BMQ
CSB-MP811390BMQ
CSB-EP811390BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Probable potassium transport system protein kup (kup), partial CSB-YP811391BMQ
CSB-EP811391BMQ
CSB-BP811391BMQ
CSB-MP811391BMQ
CSB-EP811391BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Urease accessory protein UreF 2 (ureF2) CSB-YP811392BMQ
CSB-EP811392BMQ
CSB-BP811392BMQ
CSB-MP811392BMQ
CSB-EP811392BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Urease subunit gamma 2 (ureA2) CSB-YP811393BMQ
CSB-EP811393BMQ
CSB-BP811393BMQ
CSB-MP811393BMQ
CSB-EP811393BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP811394BMQ
CSB-EP811394BMQ
CSB-BP811394BMQ
CSB-MP811394BMQ
CSB-EP811394BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 30S ribosomal protein S17 (rpsQ) CSB-YP811395BMQ
CSB-EP811395BMQ
CSB-BP811395BMQ
CSB-MP811395BMQ
CSB-EP811395BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 50S ribosomal protein L5 (rplE) CSB-YP811396BMQ
CSB-EP811396BMQ
CSB-BP811396BMQ
CSB-MP811396BMQ
CSB-EP811396BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Ribosome-recycling factor (frr) CSB-YP811397BMQ
CSB-EP811397BMQ
CSB-BP811397BMQ
CSB-MP811397BMQ
CSB-EP811397BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Putative membrane protein insertion efficiency factor (BR1073, BS1330_I1069) CSB-YP811398BMQ
CSB-EP811398BMQ
CSB-BP811398BMQ
CSB-MP811398BMQ
CSB-EP811398BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Glutamate--tRNA ligase 1 (gltX1) CSB-YP811399BMQ
CSB-EP811399BMQ
CSB-BP811399BMQ
CSB-MP811399BMQ
CSB-EP811399BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Molybdenum cofactor guanylyltransferase (mobA) CSB-YP811400BMQ
CSB-EP811400BMQ
CSB-BP811400BMQ
CSB-MP811400BMQ
CSB-EP811400BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Trigger factor (tig) CSB-YP811401BMQ
CSB-EP811401BMQ
CSB-BP811401BMQ
CSB-MP811401BMQ
CSB-EP811401BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP811402BMQ
CSB-EP811402BMQ
CSB-BP811402BMQ
CSB-MP811402BMQ
CSB-EP811402BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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