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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP812126VFI
CSB-EP812126VFI
CSB-BP812126VFI
CSB-MP812126VFI
CSB-EP812126VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0250 protein VV1_0282 (VV1_0282) CSB-YP812127VFI
CSB-EP812127VFI
CSB-BP812127VFI
CSB-MP812127VFI
CSB-EP812127VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP812128VFI
CSB-EP812128VFI
CSB-BP812128VFI
CSB-MP812128VFI
CSB-EP812128VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0319 protein VV1_0237 (VV1_0237) CSB-YP812129VFI
CSB-EP812129VFI
CSB-BP812129VFI
CSB-MP812129VFI
CSB-EP812129VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adenylate kinase (adk) CSB-YP812130VFI
CSB-EP812130VFI
CSB-BP812130VFI
CSB-MP812130VFI
CSB-EP812130VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0207 protein VV1_0013 (VV1_0013) CSB-YP812131VFI
CSB-EP812131VFI
CSB-BP812131VFI
CSB-MP812131VFI
CSB-EP812131VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus DNA replication and repair protein recF (recF) CSB-YP812132TDQ
CSB-EP812132TDQ
CSB-BP812132TDQ
CSB-MP812132TDQ
CSB-EP812132TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Bifunctional NAD (P)H-hydrate repair enzyme Nnr (nnr), partial CSB-YP812133TDQ
CSB-EP812133TDQ
CSB-BP812133TDQ
CSB-MP812133TDQ
CSB-EP812133TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) CSB-YP812134TDQ
CSB-EP812134TDQ
CSB-BP812134TDQ
CSB-MP812134TDQ
CSB-EP812134TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP812135TDQ
CSB-EP812135TDQ
CSB-BP812135TDQ
CSB-MP812135TDQ
CSB-EP812135TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus CinA-like protein (tlr2129) CSB-YP812136TDQ
CSB-EP812136TDQ
CSB-BP812136TDQ
CSB-MP812136TDQ
CSB-EP812136TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP812137TDQ
CSB-EP812137TDQ
CSB-BP812137TDQ
CSB-MP812137TDQ
CSB-EP812137TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Cell division topological specificity factor (minE) CSB-YP812138TDQ
CSB-EP812138TDQ
CSB-BP812138TDQ
CSB-MP812138TDQ
CSB-EP812138TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus SsrA-binding protein (smpB) CSB-YP812139TDQ
CSB-EP812139TDQ
CSB-BP812139TDQ
CSB-MP812139TDQ
CSB-EP812139TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Acyl carrier protein (acpP) CSB-YP812140TDQ
CSB-EP812140TDQ
CSB-BP812140TDQ
CSB-MP812140TDQ
CSB-EP812140TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus NAD (P)H-quinone oxidoreductase chain 4 2 (ndhD2), partial CSB-YP812141TDQ
CSB-EP812141TDQ
CSB-BP812141TDQ
CSB-MP812141TDQ
CSB-EP812141TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP812142TDQ
CSB-EP812142TDQ
CSB-BP812142TDQ
CSB-MP812142TDQ
CSB-EP812142TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Probable cytosol aminopeptidase (pepA) CSB-YP812143TDQ
CSB-EP812143TDQ
CSB-BP812143TDQ
CSB-MP812143TDQ
CSB-EP812143TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP812144TDQ
CSB-EP812144TDQ
CSB-BP812144TDQ
CSB-MP812144TDQ
CSB-EP812144TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Elongation factor Ts (tsf) CSB-YP812145TDQ
CSB-EP812145TDQ
CSB-BP812145TDQ
CSB-MP812145TDQ
CSB-EP812145TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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