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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Erwinia tasmaniensis Methionyl-tRNA formyltransferase (fmt) CSB-YP015150ENB
CSB-EP015150ENB
CSB-BP015150ENB
CSB-MP015150ENB
CSB-EP015150ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrenophora tritici-repentis Cytoplasmic tRNA 2-thiolation protein 2 (ncs2) CSB-YP003313FHT
CSB-EP003313FHT
CSB-BP003313FHT
CSB-MP003313FHT
CSB-EP003313FHT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrenophora tritici-repentis Molybdopterin synthase catalytic subunit (mocs2) CSB-YP014706FHT
CSB-EP014706FHT
CSB-BP014706FHT
CSB-MP014706FHT
CSB-EP014706FHT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ubiquitin-related modifier 1 homolog (urm-1) CSB-YP025676CXY
CSB-EP025676CXY
CSB-BP025676CXY
CSB-MP025676CXY
CSB-EP025676CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Malate dehydrogenase (mdh) CSB-YP013623WBO
CSB-EP013623WBO
CSB-BP013623WBO
CSB-MP013623WBO
CSB-EP013623WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Ribonuclease 3 (rnc) CSB-YP019809WBO
CSB-EP019809WBO
CSB-BP019809WBO
CSB-MP019809WBO
CSB-EP019809WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Ribosome-recycling factor (frr) CSB-YP014941WBO
CSB-EP014941WBO
CSB-BP014941WBO
CSB-MP014941WBO
CSB-EP014941WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Peptide chain release factor 1 (prfA) CSB-YP015197OEV
CSB-EP015197OEV
CSB-BP015197OEV
CSB-MP015197OEV
CSB-EP015197OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Serine hydroxymethyltransferase (glyA) CSB-YP021274OEV
CSB-EP021274OEV
CSB-BP021274OEV
CSB-MP021274OEV
CSB-EP021274OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Teashirt homolog 1 (tshz1), partial CSB-YP025135DIL
CSB-EP025135DIL
CSB-BP025135DIL
CSB-MP025135DIL
CSB-EP025135DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein arginine N-methyltransferase 2 (prmt2) CSB-YP018732XBF
CSB-EP018732XBF
CSB-BP018732XBF
CSB-MP018732XBF
CSB-EP018732XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum tRNA pseudouridine synthase A (truA) CSB-YP019086BTA
CSB-EP019086BTA
CSB-BP019086BTA
CSB-MP019086BTA
CSB-EP019086BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylacidiphilum infernorum Elongation factor Ts (tsf) CSB-YP025124MSV
CSB-EP025124MSV
CSB-BP025124MSV
CSB-MP025124MSV
CSB-EP025124MSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter lovleyi ATP synthase subunit beta (atpD) CSB-YP002350GFO
CSB-EP002350GFO
CSB-BP002350GFO
CSB-MP002350GFO
CSB-EP002350GFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Ribosome-recycling factor (frr) CSB-YP014941DSR
CSB-EP014941DSR
CSB-BP014941DSR
CSB-MP014941DSR
CSB-EP014941DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium limicola Glycine cleavage system H protein (gcvH) CSB-YP009335DSR
CSB-EP009335DSR
CSB-BP009335DSR
CSB-MP009335DSR
CSB-EP009335DSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Argininosuccinate synthase (argG) CSB-YP002234DSS
CSB-EP002234DSS
CSB-BP002234DSS
CSB-MP002234DSS
CSB-EP002234DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Ribosome-recycling factor (frr) CSB-YP014941DSS
CSB-EP014941DSS
CSB-BP014941DSS
CSB-MP014941DSS
CSB-EP014941DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917DSS
CSB-EP022917DSS
CSB-BP022917DSS
CSB-MP022917DSS
CSB-EP022917DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Malate dehydrogenase (mdh) CSB-YP013623DSS
CSB-EP013623DSS
CSB-BP013623DSS
CSB-MP013623DSS
CSB-EP013623DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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