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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Helicobacter pylori Acyl carrier protein (acpP) CSB-YP015636HUY
CSB-EP015636HUY
CSB-BP015636HUY
CSB-MP015636HUY
CSB-EP015636HUY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Ferrochelatase (hemH) CSB-YP008579HUY
CSB-EP008579HUY
CSB-BP008579HUY
CSB-MP008579HUY
CSB-EP008579HUY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Methylthioribose-1-phosphate isomerase (mri1) CSB-YP014799SXT
CSB-EP014799SXT
CSB-BP014799SXT
CSB-MP014799SXT
CSB-EP014799SXT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Branchiostoma floridae BRCA1-A complex subunit BRE (BRE) CSB-YP002808BVY
CSB-EP002808BVY
CSB-BP002808BVY
CSB-MP002808BVY
CSB-EP002808BVY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Flap endonuclease 1 (fen1) CSB-YP008585ETM
CSB-EP008585ETM
CSB-BP008585ETM
CSB-MP008585ETM
CSB-EP008585ETM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papio hamadryas Sperm acrosome membrane-associated protein 3 (SPACA3), partial CSB-YP022454ERJ
CSB-BP022454ERJ
CSB-MP022454ERJ
CSB-EP022454ERJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Translation initiation factor IF-3 (infC) CSB-YP015166DPP
CSB-EP015166DPP
CSB-BP015166DPP
CSB-MP015166DPP
CSB-EP015166DPP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methionyl-tRNA formyltransferase (fmt) CSB-YP015150DPP
CSB-EP015150DPP
CSB-BP015150DPP
CSB-MP015150DPP
CSB-EP015150DPP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triosephosphate isomerase (tpiA) CSB-YP024102DPP
CSB-EP024102DPP
CSB-BP024102DPP
CSB-MP024102DPP
CSB-EP024102DPP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Flap endonuclease 1 (fen) CSB-YP008585TMR
CSB-EP008585TMR
CSB-BP008585TMR
CSB-MP008585TMR
CSB-EP008585TMR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Draxin CSB-YP003559CH
CSB-EP003559CH
CSB-BP003559CH
CSB-MP003559CH
CSB-EP003559CH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Glycine cleavage system H protein (gcvH) CSB-YP009335BZO
CSB-EP009335BZO
CSB-BP009335BZO
CSB-MP009335BZO
CSB-EP009335BZO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Thymidylate kinase (tmk) CSB-YP007226BZO
CSB-EP007226BZO
CSB-BP007226BZO
CSB-MP007226BZO
CSB-EP007226BZO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum subsp. infantis ATP synthase subunit alpha (atpA), partial CSB-YP002344BTB
CSB-EP002344BTB
CSB-BP002344BTB
CSB-MP002344BTB
CSB-EP002344BTB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146AWN
CSB-EP019146AWN
CSB-BP019146AWN
CSB-MP019146AWN
CSB-EP019146AWN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Elongation factor Ts (tsf) CSB-YP025124AWN
CSB-EP025124AWN
CSB-BP025124AWN
CSB-MP025124AWN
CSB-EP025124AWN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Translation initiation factor IF-2 (infB), partial CSB-YP015164AWN
CSB-EP015164AWN
CSB-BP015164AWN
CSB-MP015164AWN
CSB-EP015164AWN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 3-ketoacyl-CoA thiolase (fadA) CSB-YP001117AWN
CSB-EP001117AWN
CSB-BP001117AWN
CSB-MP001117AWN
CSB-EP001117AWN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Elongation factor Tu (tuf) CSB-YP025342AWN
CSB-EP025342AWN
CSB-BP025342AWN
CSB-MP025342AWN
CSB-EP025342AWN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Glycine cleavage system H protein (gcvH) CSB-YP009335AWN
CSB-EP009335AWN
CSB-BP009335AWN
CSB-MP009335AWN
CSB-EP009335AWN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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