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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Litoria rubella Electrin-1 CSB-YP305950LHR
CSB-EP305950LHR
CSB-BP305950LHR
CSB-MP305950LHR
CSB-EP305950LHR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litoria splendida Caerin-1.10 CSB-YP305951LHS
CSB-EP305951LHS
CSB-BP305951LHS
CSB-MP305951LHS
CSB-EP305951LHS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Blaberus craniifer Cuticle protein 5 CSB-YP305952BHA
CSB-EP305952BHA
CSB-BP305952BHA
CSB-MP305952BHA
CSB-EP305952BHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP305953DWY
CSB-EP305953DWY
CSB-BP305953DWY
CSB-MP305953DWY
CSB-EP305953DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloydius brevicaudus siniticus Phospholipase A2 inhibitor subunit gamma A CSB-YP305954GGP
CSB-EP305954GGP
CSB-BP305954GGP
CSB-MP305954GGP
CSB-EP305954GGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cydia pomonella Cydiastatin-2 CSB-YP305955CVH
CSB-EP305955CVH
CSB-BP305955CVH
CSB-MP305955CVH
CSB-EP305955CVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cydia pomonella Cydiastatin-7 CSB-YP305956CVH
CSB-EP305956CVH
CSB-BP305956CVH
CSB-MP305956CVH
CSB-EP305956CVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Locusta migratoria Cuticle protein 9.5 CSB-YP305957LHY
CSB-EP305957LHY
CSB-BP305957LHY
CSB-MP305957LHY
CSB-EP305957LHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori Unknown protein 4 from 2D-page CSB-YP305958BTT
CSB-EP305958BTT
CSB-BP305958BTT
CSB-MP305958BTT
CSB-EP305958BTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori Unknown protein 19 from 2D-PAGE CSB-YP305959BTT
CSB-EP305959BTT
CSB-BP305959BTT
CSB-MP305959BTT
CSB-EP305959BTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viola odorata Cycloviolacin-O1 CSB-YP305960VDB
CSB-EP305960VDB
CSB-BP305960VDB
CSB-MP305960VDB
CSB-EP305960VDB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss Salmocidin-2B CSB-YP305961OEI
CSB-EP305961OEI
CSB-BP305961OEI
CSB-MP305961OEI
CSB-EP305961OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea 50S ribosomal protein L31, chloroplastic (RPL31) CSB-YP305962FKI
CSB-EP305962FKI
CSB-BP305962FKI
CSB-MP305962FKI
CSB-EP305962FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable 125 kDa kinesin-related protein (At2g36200), partial CSB-YP305963DOA
CSB-EP305963DOA
CSB-BP305963DOA
CSB-MP305963DOA
CSB-EP305963DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana AP2/ERF and B3 domain-containing transcription repressor RAV2 (RAV2) CSB-YP305964DOA
CSB-EP305964DOA
CSB-BP305964DOA
CSB-MP305964DOA
CSB-EP305964DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homarus americanus Hemocyanin subunit 2 CSB-YP305965HWO
CSB-EP305965HWO
CSB-BP305965HWO
CSB-MP305965HWO
CSB-EP305965HWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maia squinado Hemocyanin subunit 1 CSB-YP305966MBF
CSB-EP305966MBF
CSB-BP305966MBF
CSB-MP305966MBF
CSB-EP305966MBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hoplosternum littorale Hemoglobin cathodic subunit alpha CSB-YP305967HHW
CSB-EP305967HHW
CSB-BP305967HHW
CSB-MP305967HHW
CSB-EP305967HHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Unknown protein from spot 107 of 2D-PAGE of thylakoid CSB-YP305968EWE
CSB-EP305968EWE
CSB-BP305968EWE
CSB-MP305968EWE
CSB-EP305968EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Unknown protein from spot 118 of 2D-PAGE of thylakoid CSB-YP305969EWE
CSB-EP305969EWE
CSB-BP305969EWE
CSB-MP305969EWE
CSB-EP305969EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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