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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Naja kaouthia Thaicobrin CSB-YP307296NAF
CSB-EP307296NAF
CSB-BP307296NAF
CSB-MP307296NAF
CSB-EP307296NAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Olisthodiscus luteus Histone H2A CSB-YP307297OBA
CSB-EP307297OBA
CSB-BP307297OBA
CSB-MP307297OBA
CSB-EP307297OBA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Major heat shock 70 kDa protein Aa (Hsp70Aa), partial CSB-YP307298DLU
CSB-EP307298DLU
CSB-BP307298DLU
CSB-MP307298DLU
CSB-EP307298DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Unknown endosperm protein B CSB-YP307299HWQ
CSB-EP307299HWQ
CSB-BP307299HWQ
CSB-MP307299HWQ
CSB-EP307299HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Regakine-1 CSB-YP307300BO
CSB-EP307300BO
CSB-BP307300BO
CSB-MP307300BO
CSB-EP307300BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prunus dulcis Seed allergenic protein 2 CSB-YP307301EZJ
CSB-EP307301EZJ
CSB-BP307301EZJ
CSB-MP307301EZJ
CSB-EP307301EZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Didelphis marsupialis Venom metalloproteinase inhibitor DM43 CSB-YP307302DDX
CSB-EP307302DDX
CSB-BP307302DDX
CSB-MP307302DDX
CSB-EP307302DDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ophiophagus hannah Protease inhibitor CSB-YP307303OEL
CSB-EP307303OEL
CSB-BP307303OEL
CSB-MP307303OEL
CSB-EP307303OEL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD (ampD) CSB-YP307304DTO
CSB-EP307304DTO
CSB-BP307304DTO
CSB-MP307304DTO
CSB-EP307304DTO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Putative odorant receptor 65a (Or65a), partial CSB-YP307305DLU1
CSB-EP307305DLU1
CSB-BP307305DLU1
CSB-MP307305DLU1
CSB-EP307305DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Putative odorant receptor 82a (Or82a), partial CSB-YP307306DLU1
CSB-EP307306DLU1
CSB-BP307306DLU1
CSB-MP307306DLU1
CSB-EP307306DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. Glutathione S-transferase CSB-YP307307PVK
CSB-EP307307PVK
CSB-BP307307PVK
CSB-MP307307PVK
CSB-EP307307PVK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis Bacteriocin lactococcin MMFII CSB-YP307308LNI
CSB-EP307308LNI
CSB-BP307308LNI
CSB-MP307308LNI
CSB-EP307308LNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scyliorhinus canicula 13.2 kDa protein CSB-YP307309SYA
CSB-EP307309SYA
CSB-BP307309SYA
CSB-MP307309SYA
CSB-EP307309SYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cerastes cerastes Disintegrin CC8B CSB-YP307310CGI
CSB-EP307310CGI
CSB-BP307310CGI
CSB-MP307310CGI
CSB-EP307310CGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombina maxima Kininogen-2 CSB-YP307311BIA
CSB-EP307311BIA
CSB-BP307311BIA
CSB-MP307311BIA
CSB-EP307311BIA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Probable FKBP-type peptidyl-prolyl cis-trans isomerase 2, chloroplastic CSB-YP307312FKI
CSB-EP307312FKI
CSB-BP307312FKI
CSB-MP307312FKI
CSB-EP307312FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 88 kDa protein CSB-YP307313BQJ
CSB-EP307313BQJ
CSB-BP307313BQJ
CSB-MP307313BQJ
CSB-EP307313BQJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombina maxima Maximins 2/H1 CSB-YP307314BIA
CSB-EP307314BIA
CSB-BP307314BIA
CSB-MP307314BIA
CSB-EP307314BIA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Thylakoid lumenal 20 kDa protein CSB-YP307315FKI
CSB-EP307315FKI
CSB-BP307315FKI
CSB-MP307315FKI
CSB-EP307315FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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