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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae Sporulation-specific protein 22 (SPS22) CSB-YP326541SVG
CSB-EP326541SVG
CSB-BP326541SVG
CSB-MP326541SVG
CSB-EP326541SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Intracellular protein transport protein USO1 (USO1), partial CSB-YP326542SVG
CSB-EP326542SVG
CSB-BP326542SVG
CSB-MP326542SVG
CSB-EP326542SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leu/Ile/Val-binding protein (livJ) CSB-YP326543DTO
CSB-EP326543DTO
CSB-BP326543DTO
CSB-MP326543DTO
CSB-EP326543DTO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Proteasome component PUP3 (PUP3) CSB-YP326544SVG
CSB-EP326544SVG
CSB-BP326544SVG
CSB-MP326544SVG
CSB-EP326544SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribulose bisphosphate carboxylase small chain (rbcS) CSB-YP326545ALT
CSB-EP326545ALT
CSB-BP326545ALT
CSB-MP326545ALT
CSB-EP326545ALT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human parainfluenza 2 virus Fusion glycoprotein F0 (F), partial CSB-YP326546HPA
CSB-EP326546HPA
CSB-BP326546HPA
CSB-MP326546HPA
CSB-EP326546HPA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage P2 Terminase, ATPase subunit (P), partial CSB-YP326547EDE
CSB-EP326547EDE
CSB-BP326547EDE
CSB-MP326547EDE
CSB-EP326547EDE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein E7 (E7) CSB-YP326548HNA
CSB-EP326548HNA
CSB-BP326548HNA
CSB-MP326548HNA
CSB-EP326548HNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrophis cyanocinctus Short neurotoxin 1 CSB-YP326549HQZ
CSB-EP326549HQZ
CSB-BP326549HQZ
CSB-MP326549HQZ
CSB-EP326549HQZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine leukemia virus Envelope glycoprotein (env), partial CSB-YP326550BKE
CSB-EP326550BKE
CSB-BP326550BKE
CSB-MP326550BKE
CSB-EP326550BKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Placobdella ornata Ornatin-A3 CSB-YP326551PLA
CSB-EP326551PLA
CSB-BP326551PLA
CSB-MP326551PLA
CSB-EP326551PLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0758 protein yicR (yicR) CSB-YP326552ENV
CSB-EP326552ENV
CSB-BP326552ENV
CSB-MP326552ENV
CSB-EP326552ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional regulator CbbR (cbbR) CSB-YP326553XAC
CSB-EP326553XAC
CSB-BP326553XAC
CSB-MP326553XAC
CSB-EP326553XAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Bud site selection protein 3 (BUD3), partial CSB-YP326554SVG
CSB-EP326554SVG
CSB-BP326554SVG
CSB-MP326554SVG
CSB-EP326554SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Peptide methionine sulfoxide reductase 2 (MXR2) CSB-YP326555SVG
CSB-EP326555SVG
CSB-BP326555SVG
CSB-MP326555SVG
CSB-EP326555SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ER membrane protein complex subunit 1 (EMC1), partial CSB-YP326556SVG
CSB-EP326556SVG
CSB-BP326556SVG
CSB-MP326556SVG
CSB-EP326556SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae AdoMet-dependent rRNA methyltransferase SPB1 (SPB1), partial CSB-YP326557SVG
CSB-EP326557SVG
CSB-BP326557SVG
CSB-MP326557SVG
CSB-EP326557SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative transposon Ty5-1 protein YCL075W (TY5B) CSB-YP326558SVG
CSB-EP326558SVG
CSB-BP326558SVG
CSB-MP326558SVG
CSB-EP326558SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 30 kDa heat shock protein (HSP30), partial CSB-YP326559SVG1
CSB-EP326559SVG1
CSB-BP326559SVG1
CSB-MP326559SVG1
CSB-EP326559SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative methyltransferase BUD23 (BUD23) CSB-YP326560SVG
CSB-EP326560SVG
CSB-BP326560SVG
CSB-MP326560SVG
CSB-EP326560SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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