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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae Uncharacterized transporter YHL008C (YHL008C), partial CSB-YP327884SVG1
CSB-EP327884SVG1
CSB-BP327884SVG1
CSB-MP327884SVG1
CSB-EP327884SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YHR003C (YHR003C) CSB-YP327885SVG
CSB-EP327885SVG
CSB-BP327885SVG
CSB-MP327885SVG
CSB-EP327885SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ATP-dependent helicase RRM3 (RRM3), partial CSB-YP327886SVG
CSB-EP327886SVG
CSB-BP327886SVG
CSB-MP327886SVG
CSB-EP327886SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ribosome-recycling factor, mitochondrial (RRF1) CSB-YP327887SVG
CSB-EP327887SVG
CSB-BP327887SVG
CSB-MP327887SVG
CSB-EP327887SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 2-deoxyglucose-6-phosphate phosphatase 2 (DOG2) CSB-YP327888SVG
CSB-EP327888SVG
CSB-BP327888SVG
CSB-MP327888SVG
CSB-EP327888SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Family of serine hydrolases 1 (FSH1) CSB-YP327889SVG
CSB-EP327889SVG
CSB-BP327889SVG
CSB-MP327889SVG
CSB-EP327889SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ribosome-associated complex subunit SSZ1 (SSZ1), partial CSB-YP327890SVG
CSB-EP327890SVG
CSB-BP327890SVG
CSB-MP327890SVG
CSB-EP327890SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YHR078W (YHR078W), partial CSB-YP327891SVG1
CSB-EP327891SVG1
CSB-BP327891SVG1
CSB-MP327891SVG1
CSB-EP327891SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Pre-rRNA-processing protein IPI1 (IPI1) CSB-YP327892SVG
CSB-EP327892SVG
CSB-BP327892SVG
CSB-MP327892SVG
CSB-EP327892SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Chromatin modification-related protein YNG2 (YNG2) CSB-YP327893SVG
CSB-EP327893SVG
CSB-BP327893SVG
CSB-MP327893SVG
CSB-EP327893SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transcription-associated protein 1 (TRA1), partial CSB-YP327894SVG
CSB-EP327894SVG
CSB-BP327894SVG
CSB-MP327894SVG
CSB-EP327894SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YHR125W (YHR125W) CSB-YP327895SVG
CSB-EP327895SVG
CSB-BP327895SVG
CSB-MP327895SVG
CSB-EP327895SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein NSG1 (NSG1), partial CSB-YP327896SVG1
CSB-EP327896SVG1
CSB-BP327896SVG1
CSB-MP327896SVG1
CSB-EP327896SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Aromatic amino acid aminotransferase 2 (ARO9), partial CSB-YP327897SVG
CSB-EP327897SVG
CSB-BP327897SVG
CSB-MP327897SVG
CSB-EP327897SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cruciform DNA-recognizing protein 1 (CRP1) CSB-YP327898SVG
CSB-EP327898SVG
CSB-BP327898SVG
CSB-MP327898SVG
CSB-EP327898SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Regulator of Ty1 transposition protein 107 (RTT107), partial CSB-YP327899SVG
CSB-EP327899SVG
CSB-BP327899SVG
CSB-MP327899SVG
CSB-EP327899SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Topoisomerase I damage affected protein 11 (TDA11), partial CSB-YP327900SVG
CSB-EP327900SVG
CSB-BP327900SVG
CSB-MP327900SVG
CSB-EP327900SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA replication ATP-dependent helicase DNA2 (DNA2), partial CSB-YP327901SVG
CSB-EP327901SVG
CSB-BP327901SVG
CSB-MP327901SVG
CSB-EP327901SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YHR173C (YHR173C) CSB-YP327902SVG
CSB-EP327902SVG
CSB-BP327902SVG
CSB-MP327902SVG
CSB-EP327902SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YHR180W (YHR180W), partial CSB-YP327903SVG
CSB-EP327903SVG
CSB-BP327903SVG
CSB-MP327903SVG
CSB-EP327903SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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