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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Schizosaccharomyces pombe ATP phosphoribosyltransferase (his1) CSB-YP328105SXV
CSB-EP328105SXV
CSB-BP328105SXV
CSB-MP328105SXV
CSB-EP328105SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Cyclin-dependent kinase inhibitor rum1 (rum1) CSB-YP328106SXV
CSB-EP328106SXV
CSB-BP328106SXV
CSB-MP328106SXV
CSB-EP328106SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Meiotic recombination protein rec6 (rec6) CSB-YP328107SXV
CSB-EP328107SXV
CSB-BP328107SXV
CSB-MP328107SXV
CSB-EP328107SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (tps1), partial CSB-YP328108SXV
CSB-EP328108SXV
CSB-BP328108SXV
CSB-MP328108SXV
CSB-EP328108SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Ras-related protein RIC2 (RIC2) CSB-YP328109OFG
CSB-EP328109OFG
CSB-BP328109OFG
CSB-MP328109OFG
CSB-EP328109OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized oxidoreductase yhxC (yhxC) CSB-YP328110BRJ
CSB-EP328110BRJ
CSB-BP328110BRJ
CSB-MP328110BRJ
CSB-EP328110BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Alkanesulfonate monooxygenase (ssuD) CSB-YP328111BRJ
CSB-EP328111BRJ
CSB-BP328111BRJ
CSB-MP328111BRJ
CSB-EP328111BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized lipoprotein ybbD (ybbD), partial CSB-YP328112BRJ
CSB-EP328112BRJ
CSB-BP328112BRJ
CSB-MP328112BRJ
CSB-EP328112BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Regulatory protein SWI6 (SWI6), partial CSB-YP328113KBK
CSB-EP328113KBK
CSB-BP328113KBK
CSB-MP328113KBK
CSB-EP328113KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Homeobox protein extradenticle (exd) CSB-YP328114DLU
CSB-EP328114DLU
CSB-BP328114DLU
CSB-MP328114DLU
CSB-EP328114DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative nitrilase-like protein YIL165C (YIL165C) CSB-YP328115SVG
CSB-EP328115SVG
CSB-BP328115SVG
CSB-MP328115SVG
CSB-EP328115SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cytochrome oxidase assembly protein 1 (COA1), partial CSB-YP328116SVG1
CSB-EP328116SVG1
CSB-BP328116SVG1
CSB-MP328116SVG1
CSB-EP328116SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YIL151C (YIL151C), partial CSB-YP328117SVG
CSB-EP328117SVG
CSB-BP328117SVG
CSB-MP328117SVG
CSB-EP328117SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein VHS2 (VHS2) CSB-YP328118SVG
CSB-EP328118SVG
CSB-BP328118SVG
CSB-MP328118SVG
CSB-EP328118SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial FAD carrier protein FLX1 (FLX1), partial CSB-YP328119SVG1
CSB-EP328119SVG1
CSB-BP328119SVG1
CSB-MP328119SVG1
CSB-EP328119SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA repair/transcription protein MET18/MMS19 (MET18), partial CSB-YP328120SVG
CSB-EP328120SVG
CSB-BP328120SVG
CSB-MP328120SVG
CSB-EP328120SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Pheromone-regulated membrane protein 5 (PRM5), partial CSB-YP328121SVG1
CSB-EP328121SVG1
CSB-BP328121SVG1
CSB-MP328121SVG1
CSB-EP328121SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein HOS4 (HOS4), partial CSB-YP328122SVG
CSB-EP328122SVG
CSB-BP328122SVG
CSB-MP328122SVG
CSB-EP328122SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Phosphatidylinositol 4,5-bisphosphate-binding protein SLM1 (SLM1), partial CSB-YP328123SVG
CSB-EP328123SVG
CSB-BP328123SVG
CSB-MP328123SVG
CSB-EP328123SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YIL100W (YIL100W) CSB-YP328124SVG
CSB-EP328124SVG
CSB-BP328124SVG
CSB-MP328124SVG
CSB-EP328124SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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